圆弧青霉提取物对枯草杆菌芽胞DNA重组效应  

The study of rec-assay ot the extracts from P.Cyclopium on Bacillus subtilis spores

作　　者：马远方[1] 甄应中[1] 周刚[1] 徐岷[1] 宋爱云[1] 徐友梅[1] 刘桂亭[1] 

机构地区：[1]河南医科大学食管癌研究所

出　　处：《河南医科大学学报》1990年第2期132-136,共5页Journal of Henan Medical University

摘　　要：从河南食管癌高发区林县粮食中分离出24株圆弧青霉,采用Raulin-Thom液体培养基培养,经三氯甲烷萃取后得棕黄色粉末状提取物。用枯草杆菌芽胞DNA重组试验检测其重组效应。结果表明:不加(S-9)活化系统16株提取物为阳性(64%),加S-9后24株皆为阴性,提示提取物中含有直接诱变物。24 strains of Penicillum Cyclopium Westling were isolated from the grain inLin Xian County of Henan Province, the area of high incidence of esophageal cancer.The strains were inoculated in Raulin-Thom fluid medium. The brownpowdery extract was extracted with chlorofrom from the condensed culturedfluid. The extracts were tested for DNA-damaging ability in the Rec-assay byusing the recmbination-deficient mutant of Bacillus Subtilis M45 and the parentstrain H 17 with their spores. 16 of 24 strains of P. Cyclopium were positive inthis system without metabolic activation. 24 strains were negative with metabolic activation.

关 键 词：圆弧青霉 食管癌 重组修复试验 

分 类 号：R735.102[医药卫生—肿瘤]