不同代次成人正常髓核细胞的形态及生长动力学比较  

Comparison on morphology and growth kinetics of adult normal nucleus pulposus cells in different passages

作　　者：张荣峰[1] 阮狄克[2] 张超[2] 孙海燕[1] 易红蕾[1] 王爱兰[1] 

机构地区：[1]潍坊市解放军第89医院脊柱外科,山东261000 [2]北京海军总医院骨科

出　　处：《脊柱外科杂志》2008年第3期137-140,共4页Journal of Spinal Surgery

基　　金：国家自然科学基金资助项目(30370389)

摘　　要：目的比较不同代次成人正常髓核细胞体外培养的形态及生长动力学差异。方法从成人正常髓核组织分离培养髓核细胞,传代后对细胞形态、生长曲线、噻唑蓝(methyl thiazolyl tetrazolium,MTT)摄取等进行比较研究。结果体外培养条件下,传3代之前的细胞形态正常,胞浆丰富;传4代起,部分细胞的形态逐渐向长梭形演化。生长曲线提示传3代之前的髓核细胞持续增殖能力强。MTT测定吸光度值,传1、3代时差异无统计学意义(P>0.05),传5、7代与传1代相比,差异具有统计学意义(P<0.05)。结论体外培养条件下,前3代成人正常髓核细胞形态良好,活性高,增殖能力强,适合作为组织工程椎间盘的种子细胞。Objective To investigate the morphology and growth kinetics of different passages cells derived from the adult normal nucleus pulposus. Methods The cells derived from the adult normal nucleus pulposus were cultured in vitro. A comparative study was performed on cell morphology, growth curve and MTI＇ assay after passaged. Results Cultured in vitro, cells before passage 3 had normal morphology and abundant cytoplasm. Some cells evolved to a long-spindle shape gradually from passage 4. Powerful continuous proliferation was revealed by growth curve before passage 3. The difference of optical density of MTI＇ assay at passage 1 and 3 didn＇t have statistical significance （P 〉 0.05 ）. The difference of passages 5 and 7 had statistical significance compared with passage 1 （ P 〈 0.05 ）. Conclusion The cells of adult normal nucleus pulposus before passage 3 could act as seed-cells of tissue-engineering intervertebral disc for their normal morphology and powerful proliferation.

关 键 词：椎间盘 细胞培养技术 组织工程 

分 类 号：R394.26[医药卫生—医学遗传学]