阿魏酸钠对大鼠角膜新生血管的抑制作用研究  

Inhibition of rat corneal neovascularization by inhibitor of sodium ferulate

作　　者：陈丽华[1] 江萍[1] 罗彤[1] 胡学斌[1] 莫纯坚[1] 王红俊[1] 

机构地区：[1]三峡大学第一临床医学院宜昌市中心人民医院眼视光学专科,宜昌443003

出　　处：《眼科研究》2008年第7期526-529,共4页Chinese Ophthalmic Research

摘　　要：目的探讨阿魏酸钠(SF)对角膜新生血管(CNV)的抑制作用及其机制。方法将不同质量浓度的SF用于缝线诱导的大鼠CNV模型,观察其抑制CNV形成的效果,采用免疫组织化学法和RT-PCR法检测血管内皮生长因子(VEGF)蛋白和mRNA在角膜组织中的表达水平。结果0.025%和0.1%SF治疗组CNV面积均小于阴性对照组,以后者更明显,差异均有统计学意义(P<0.05),0.1%SF治疗组与阳性对照组间差异无统计学意义(P>0.05);免疫组织化学显示VEGF在正常角膜不表达或在上皮基底细胞有弱表达,阴性对照组染色明显增强,0.1%SF治疗组表达明显减弱;VEGFmRNA和蛋白水平表达有一致性。结论SF滴眼液能有效地抑制CNV的生长,其抑制VEGF的表达可能是SF抑制CNV生成的一个重要机制。Objective Corneal neovascularization（CNV） is one of the severe complications of cornea disease. Hitherto, there are still no efficient drugs with few side effects to prevent its development. Present study was to observe the inhibiting effect of sodium ferulate（SF） on CNV and its mechanism. Methods CNV was induced by corneal suture method on 48 male Sprague-Dawley rats. The rats were randomly divided into 4 groups and 12 rats for each. 0. 025% or 0. 1% SF eye drops was respectively used in group B and group C. PBS eye drops was administed in group A as the negative control and 0.1% dexamethasone sodium phosphate eye drops for group D as the positive control. Various eye drops were applied four times daily during day 0 - 12. The CNV was observed under the slit lamp biomicroscope and the CNV area was respectively calculated at the 3rd,6th,9th and 12nd day after operation. Corneal sample was collected at the 12nd day for histopathological examination. The expression levels of VEGF mRNA and VEGF protein in cornea were evaluated by reverse transcription-polymerase chain reaction （RT-PCR） and immunohistochemistry assay. Results The area of CNV was significantly decreased in group B, C and D compared with group A （P 〈 0. 05 ） ,and that in group C and D was smaller than group B （P 〈 0.01 ） in various time points, but no significant difference between group C and group D （ P 〉 0. 05 ）. The expression of VEGF in the basis epithelium was markedly increased in group A in comparison with group B, C and D （ P 〈 0.01 ） , and no significant difference between group C and group D （P 〉 0.05 ）. The expression of VEGF mRNA followed a similar pattern to that of VEGF protein. Conclusion Topical application of SF has significant effects on the inhibition of CNV. The mechanism of antiangiogenesis may be related to inhibition effect of SF on VEGF expression.

关 键 词：角膜新生血管 阿魏酸钠 血管内皮生长因子 

分 类 号：R772.2[医药卫生—眼科]