蛋白酶体抑制剂MG132诱导牛晶状体上皮细胞凋亡的观察  

Apoptosis of bovine lens epithelial cells induced by proteasome inhibitor MG132

作　　者：邢星[1] 胡义珍[1] 曹阳[1] 徐志蓉[1] 熊林杰[1] 

机构地区：[1]华中科技大学同济医学院附属协和医院眼科,武汉430022

出　　处：《眼科研究》2008年第7期530-532,共3页Chinese Ophthalmic Research

摘　　要：目的观察蛋白酶体抑制剂MG132对晶状体上皮细胞(LECs)凋亡的诱导作用。方法用不同浓度的MG132分别处理牛LECs12、24、36h,通过MTT法检测细胞活力,流式细胞术(FCM)检测MG132对牛LECs凋亡的影响。结果在作用时间相同的条件下,随着MG132浓度的增高(0、2、5、10μmol/L),对牛LECs增生的抑制作用逐渐增强(P<0.01)。当作用时间达36h时,半效抑制浓度IC50为2.03μmol/L。同时,MG132能够明显诱导牛LECs凋亡:FCM分析结果表明,浓度为2μmol/L的MG132作用12h,细胞早期凋亡率为20.24%±1.51%,对照组为0.98%±0.20%(P<0.01,n=3)。结论蛋白酶体抑制剂MG132能够诱导牛LECs凋亡,蛋白酶体活性的丧失可能在白内障的形成中起重要作用。Objective Ophthalmologic scholars have discovered in vitro experiments that the apoptosis of lens epithelial cells （LECs） plays an influential role in the early stage of cataract. Proteasome can hydrolyze normal and abnormal proteins and therefore are involved in the cellular mitotic cycle,apoptosis,signal transduction,etc.. This study tried to investigate the effect of proteasome inhibitor MG132 on the apoptosis of bovine lens epithelial cells. Methods Bovine lens epithelial cells were cultured and passaged in serum medium. The different concentrations of MG132 （0,2,5,10 μmol/L） was added in medium for 12,24 and 36 hours. The cellular viability was analyzed by MTT assay and the effect of MG132 on the apoptosis of bovine lens epithelial cells was analyzed by flow cytometry （FCM）. Results Treated for a same period,the inhibitory effect of MG132 on bovine lens epithelial cells proliferation enhanced with the increment of the concentration of MG132 （ P 〈 0. 01 ）. The 50% inhibiting concentration（IC50） of MG132 was 2. 03 μmol/L in 36 hours after the bovine lens epithelial cells were treated with MG132. The cellular apoptosis was also demonstrated obviously in MG132-treated bovine lens epithelial cells with the apoptosis index 20. 24% ± 1.51% in 12 hours in 2 μmol/L MG132 group by flow cytometry,and that of the control cells was 0. 98% ± 0. 20% （ t = 24.21, P 〈 0. 01 ,n = 3 ）. Conclusion MG132 can lead to apoptosis of bovine lens epithelial cells, suggesting that the decrease of the proteasome activity may play an important role in the formation and development of cataract.

关 键 词：蛋白酶体抑制剂 晶状体上皮细胞 白内障 细胞凋亡 

分 类 号：R776.1[医药卫生—眼科]