野菊花总黄酮的提取与纯化  

Extraction and purification of flavonoids from chrysanthemum indicun

作　　者：罗显华[1] 郁建生[1] 

机构地区：[1]铜仁职业技术学院,铜仁554300

出　　处：《食品科技》2008年第7期179-183,共5页Food Science and Technology

基　　金：贵州省省长专项资金项目(黔省专合字(2005)261号);贵州省"十一五"农业科技攻关项目(黔科合NY字[2006]3020)

摘　　要：以野菊花总黄酮含量及回收率等为考察指标,研究野菊花总黄酮提取工艺及大孔吸附树脂分离纯化野菊花总黄酮工艺。结果表明:采取乙醇浸提L9(34)正交试验方法,野菊花总黄酮最佳提取工艺条件为乙醇浓度60%、提取温度80℃、提取时间3h、提取次数3次。AB-8型大孔吸附树脂对野菊花总黄酮静态饱和吸附量为114.65mg/g(干树脂),洗脱率94.9%,动态饱和吸附量为94.5mg/g(干树脂),总黄酮回收率在92.6%、纯度在90%以上,是实验树脂中分离纯化野菊花总黄酮的最佳大孔吸附树脂。分离纯化野菊花总黄酮最佳工艺条件为AB-8型大孔吸附树脂,洗脱剂为70%乙醇,洗脱剂用量为3倍树脂体积,流速3～4mL/min,上柱总黄酮量与树脂比为1∶10.5,上柱液总黄酮浓度为19.8mg/mL,流速2～3mL/min,上柱液pH值4～5,冲洗杂质用水体积2～3BV。A method for extraction and purification of flavonoids from chrysanthemum indicun with macroporous resin was studied, with the content and recovery rate of flavonoids as indexes, The results are as flollows： The experiment of L9（3^4） by ethanol as extracting way, the optimum conditions of extracting flavonoids from chrysanthemum indicun are obtained, When the condition is 60% of density of ethanol, during 3 hours at 80 ℃ and extracting 3 times. The static adsorption capacity of AB-8 type resin was 114,65 mg/g, the static elution ratio were 94,9%, the dynamic adsorption capacity of AB-8 type resin was 94,5 mg/g, the recovery rate is more than 92.6% and the purity is more than 90%. AB-8 type resin is the best for separating and purificating chrysanthemum indicun in flavonoids. The optimum conditions is： the eluant is 70% alcohol and 2-3 times as the volume of the resin, the volume of the resin is 10.6 times of flavonoids in sample, concentration of flavonoids of sample is 19.8 mg/mL and current velocity of 2-3 mL/min, pH value of sample is 4-5.

关 键 词：野菊花 总黄酮 提取 纯化 

分 类 号：TS201.1[轻工技术与工程—食品科学]