骨髓间充质干细胞对人肝癌细胞系MHCC97-H增殖能力的影响  

The effect of mesenchymal stem cells on the proliferation of MHCC97-H cell line

作　　者：李国才[1] 武金才[1] 孙冰生[1] 刘道永[1] 钦伦秀[1] 

机构地区：[1]复旦大学肝癌研究所复旦大学中山医院复旦大学生物医学研究院,上海200032

出　　处：《中华实验外科杂志》2008年第7期823-825,共3页Chinese Journal of Experimental Surgery

基　　金：国家“863”计划资助项目（2006AA02Z473）;国家杰出青年基金资助项目（30325041）

摘　　要：目的观察骨髓间充质干细胞（MSC）对肝癌细胞增殖能力的影响。方法人肝癌细胞系MHCC97-H培养液中分别加入0.25％和50％MSC的条件培养基（MSC-CM），采用CyQUANT细胞增殖实验检测吸光度A值变化。在MHCC97-H细胞培养液中添加50％MSC-CM，荧光实时定量聚合酶链反应（RT-PCR）检测肿瘤增殖细胞核抗原（PCNA）、Ki-67抗原的变化。16只裸鼠皮下接种MHCC97-H细胞后，实验组经静脉注射MSC，1×10。个／次，每周3次，对照组静脉注射PBS；比较肿瘤体积。结果培养液中加入MSC-CM后A值依次为211．65±54．72、236．24±57．15和283．59±62．16（P〈0．05）。PCNA和Ki-67的表达水平分别升高1．8和7．0倍。实验组肿瘤体积（1745±455）mm^3大于对照组（972±568）mm^3（P〈0．05），肿瘤体积平均增加速度（56．0±26．1）mm^3／d高于对照组（32．4±14．5）mm3／d（P〈0．05）。结论骨髓间充质干细胞可促进肝癌细胞系MHCC97-H的增殖。Objective To investigate the possible role of mesenchymal stem cells （MSC） in the proliferation of hepatocellular carcinoma （HCC） cells. Methods The HCC cell line （MHCC-97H） was incubated with 0, 25% and 50% MSC condition medium （ MSC-CM ） respectively and tested by CyQUANT cell proliferation assay. After MHCC97-H cells were cultured with 50% MSC-CM, the expression levels of proliferating cell nuclear antigen （PCNA） and Ki67 were tested by Real time-PCR. Sixteen nude mice inoculated subcutaneously with MHCC97-H cells were treated with 1 × 10^6 MSC or PBS via in- travenous injection three times per week. The tumor sizes were evaluated. Results After MHCC97-H cells were treated with 0,25% and 50% MSC-CM, A values were 211.65 ± 54.72,236.24 ± 57. 15 and 283.59 ± 62.16 respectively （P 〈0.05 ）. PCNA and Ki-67 expression levels in MHCC97-H cells treated with MSC-CM were increased by 1.8 and 7 times, respectively. The tumor volume in MSC-treated group （1745±455） mm^3 was much bigger than that in the controls （972 ±568） mm^3 ,P 〈0.05,and also the average tumor growth rate （56.0 ±26.1 ） mm^3/day was significantly increased as compared with the controls （32.4 ± 14.5 ） mm^3/day,P 〈 0.05. Conclusion MSC can promote both in vitro proliferation and in vivo tumor growth of HCCs.

关 键 词：癌 肝细胞 间充质干细胞 增殖 

分 类 号：R735.7[医药卫生—肿瘤]