用清除有机自由基DPPH法评价竹叶提取物抗氧化能力  

Detection of Antioxidative Capacity of Bamboo Leaf Extract by Scavenging Organic Free Radical DPPH

作　　者：郭雪峰[1] 岳永德[1] 汤锋[1] 王进[1] 姚曦[1] 

机构地区：[1]国际竹藤网络中心,国家林业局竹藤科学与技术重点开放实验室,北京100102

出　　处：《光谱学与光谱分析》2008年第7期1578-1582,共5页Spectroscopy and Spectral Analysis

基　　金：国家“十一五”科技支撑项目(2006BAD19B08);国际竹藤网络中心基本科研业务费专项资金(06/07-B14)资助

摘　　要：通过对1,1-二苯基苦基苯肼(DPPH)溶液吸收光谱、DPPH溶液反应体系的研究,得出以下结论,分光光度法测定DPPH溶液反应体系的测定波长为518.4nm,反应体系为4.00mL257.7mg·L-1的DP-PH溶液中加1mL不同浓度的抗氧化剂,反应体系加入抗氧化剂后反应时间为40min;用上述方法研究评价合成抗氧化剂叔丁基对苯二酚(TBHQ)和2,6-二叔丁基对甲酚(BHT)对DPPH自由基清除率和浓度的关系,以IC50值(清除率为50%时,抗氧化剂的浓度值)作为评价指标,测得合成抗氧化剂和效果最好竹叶提取物样品IC50值分别为,TBHQ(21.14mg·L-1),BHT(42.09mg·L-1),M40(108.40mg·L-1),M40等竹叶提取物可以作为天然抗氧化剂进行开发。By studying on absorption spectrum of DPPH solution and DPPH reaction system, the conclusion was drawn as follows, the wavelength for the determination of DPPH reaction system by spectrophotometric method was chosen to be 518.4 nm, DPPH reaction system was 4.00 mL of 257.7 mg· L^-1 DPPH＋1 mL of antioxidant solution with the reaction time of DPPH reaction system of 40 min. The relation between DPPH radical scavenging rate and the concentration of TBHQ and BHT was studied, and IC50 （the value of antioxidant concentration for scavenging half of DPPH radical） was used as the index to evaluate scavenging capacity. The determining IC50 values were as follows： TBHQ（21.14 mg·L^-1 ）, BHT（42.09 mg ·L^-1） and M40 （108. 40 mg ·L^-1 ）. Bamboo leaf extract could be used as natural antioxidant.

关 键 词：竹叶提取物 1 1-二苯基苦基苯肼 清除率 IC50值 

分 类 号：TS201.2[轻工技术与工程—食品科学]