海洋红酵母耐盐cDNA文库的构建与序列分析  

Analysis Sequence and Construct of cDNA Library of Stress Salt from Ocean Rhodotorula

作　　者：刘洪博[1] 王宇光[1] 雷禄旺[1] 

机构地区：[1]中国热带农业科学院热带生物技术研究所,海口571101

出　　处：《中国农学通报》2008年第7期74-76,共3页Chinese Agricultural Science Bulletin

基　　金：国家自然科学基金资助项目(30560117);海南省科技厅资助项目

摘　　要：利用消减杂交技术(SSH)构建了海洋红酵母耐盐cDNA文库,一共挑出270个克隆子,选取了25个克隆进行测序,经过Blast分析,初步认为有一个克隆是与耐盐相关的基因,2个属于未知基因,其余为核RNA。cDNA library of stress salt from ocean Rhodotorula is constructed with subtraction hybridization, two hundred and seventy clones were picked, and twenty five clones have known sequence, was analysed from Blast, one clone related with tolerance of salt, two clones were unknown gene, others were rRNA.

关 键 词：红酵母 耐盐基因 消减杂交 

分 类 号：Q939.9[生物学—微生物学]