姜黄素抑制MMP-2及其抑制因子表达治疗肝纤维化的实验研究  

Effect of curcumin against CCl4 - induced liver fibrosis in rats

作　　者：朱宝和 黄建贤 黄林 贺德 王甘露 马广念 Zhu Baohe,Huang Jianxian,Huang Lin,He De, Wang Ganlu,Ma Guangnian. （Department of general surgery,the affiliated Bao＇an people＇s hospital of nanfang medical university,Shenzhen 518101 China）

机构地区：南方医科大学附属宝安人民医院,广东深圳518101

出　　处：《中国保健》2008年第21期925-927,共3页Chinese Health Care

摘　　要：目的：探讨姜黄素抑制肝纤维化的效应及其机制。方法：复制大鼠四氯化碳（CCl4）肝纤维化模型，分别以高、中、低剂量姜黄素处理，检测血清透明质酸（HA）、层粘连蛋白（LN）、三型前胶原（PCIII）和四型胶原（Ⅵ．C）水平，检测肝组织纤维化程度和基质金属蛋白酶-2（MMP-2）及其抑制因子组织型金属蛋白酶抑制剂-2（TIMP-2）mRNA表达。结果：CCl4成功诱导肝纤维化大鼠模型，姜黄素治疗能够降低血清肝纤维化指标及肝组织纤维化程度，姜黄素可以抑制CCl1诱导的MMP-2和TIMP-2mRNA表达，并能恢复MMP-2／TIMP-2的动态平衡。结论：姜黄素可以通过减少MMP-2及其抑制因子表达抑制肝纤维化。Objective：To investigate the curative effect and its mechanism of curcumin on liver fibrosis in rats induced by CCl4. Methods：Models of liver fibrosis in rats induced by subcutaneously injection of CCl4 were treated with curcumin ormalotilate as positive control. The levels of HA, LN, PC III and IV. C in serum and histological changes in liver tissue were determined to reveal the event liver damage. MMP - 2 and TIMP - 2 mR- NA expression in liver tissues were determined by RT - PCR. Results： Curcumin significantly reduced the levels of HA, LN, PC III and IV. C in serum, and liver fibrosis was also improved. Curcumin also inhibited MMP -2 and TIMP -2 mRNA expression in liver tissues induced by CCl4. Conclnsion. Curcumin may have therapeutic effect on liver fibrosis by inhibiting MMP -2 and TIMP -2 expression.

关 键 词：姜黄素 肝纤维化 MMP-2 TIMP-2 

分 类 号：R575.2[医药卫生—消化系统;医药卫生—临床医学;医药卫生—内科学]