体外培养兔视网膜Müller细胞葡萄糖转运载体蛋白1表达及其影响因素研究  

Study of expression and effect factors on glucose transporter-1 of rabbit retinal Müller cells cultured in vitro

作　　者：宋鄂[1] 关小康[1] 王秋利[1] 王雅坤[1] 鞠胜[1] 毕明超[1] 

机构地区：[1]吉林大学第一医院眼科,长春130021

出　　处：《中华眼底病杂志》2008年第4期265-267,共3页Chinese Journal of Ocular Fundus Diseases

基　　金：国家自然科学基金（30571992）;吉林省科技厅杰出青年基金（20030111）

摘　　要：目的 观察高糖以及高浓度胰岛素等不同因素对体外培养兔视网膜Müller细胞葡萄糖转运载体蛋白1（GLUT1）表达的影响。方法 利用组织块悬浮法原代培养兔视网膜Müller细胞，分为正常对照组、高糖组、胰岛素组、高糖加胰岛素组，通过激光共聚焦显微镜结合免疫细胞化学、荧光染色的方法，定位定量分析GLUT1的表达情况。结果 高糖及高浓度胰岛素条件下视网膜Müller细胞GLUT1的表达均明显增强，并主要位于细胞浆靠近核膜周围。结论 视网膜Müller细胞可表达GLUT1，高糖以及高浓度胰岛素均可使其表达增强。Objective To observe the different effect such as high concentration of glucose and high concentration of insulin on GLUT1 of Rabbit Retinal Müller Cell in vitro. Methods Rabbit retinal Müller cells were cultured in vitro with suspended constitution,which were divided as the following groups： common control group,high glucose group,insulin group,high glucose combined insulin group. Laser confocal microscope combined with immunocytochemical and fluorescence staining method to quantitatively analyze the expression condition of GLUT1. Results The expression of GLUT1 has been enhanced obviously by high glucose and high insulin,which locates mainly in the cytoplasm that near to the nucleus. Conclusion Rabbit retinal Müller cells can express GLUT1,and the expression of GLUT1 can be reinforced by high glucose and high insulin.

关 键 词：葡萄糖转运体1型 神经胶质 MÜLLER细胞 

分 类 号：R774.1[医药卫生—眼科] R587.1[医药卫生—临床医学]