人乳腺癌细胞系MCF-7中不同亚群细胞的体内外克隆和成瘤能力分析  

Analysis on Clone in vitro and Tumorigenic Capacity in vivo of Different Subsets Cells From the MCF-7 Human Breast Cancer Cell Line

作　　者：李治 刘春萍[1] 贺艳丽[1] 田元[1] 黄韬[1] 

机构地区：[1]华中科技大学协和医院乳腺甲状腺外科,武汉430022

出　　处：《四川大学学报（医学版）》2008年第4期547-549,共3页Journal of Sichuan University(Medical Sciences)

基　　金：国家自然科学基金(批准号30571798);湖北省十一五重大科技攻关项目(批准号2006AA301A05)资助

摘　　要：目的了解在人乳腺癌细胞系MCF-7中是否存在乳腺癌干细胞。方法采用流式细胞技术对MCF-7细胞进行分选,检测MCF-7中不同亚群细胞的体外克隆形成能力和体内成瘤能力。结果MCF-7中有部分细胞具有体外克隆形成能力和体内成瘤能力,而且和CD44+CD24+细胞相比,CD44+CD24-细胞中成瘤细胞的含量更高。结论MCF-7中存在乳腺癌干细胞,并主要集中于CD44+CD24-细胞中,CD44+CD24+细胞也可能是乳腺癌祖细胞。Objective To investigate whether there are cancer stem cells in the MCF-7 human breast cancer cell line.Methods Flow cytometry was applied to separate different subpopulation cells from MCF-7 cells,and their ability of clone in vitro and reconstruction tumor in vivo were determined.Results The ability of clone in vitro and reconstruction tumor in vivo were observed in some MCF-7 cells.Contrast with CD44^＋CD24^＋ cells,the proportion of tumorigenic cancer cells in CD44^＋CD24^-cells is higher.Conclusion Breast cancer stem cell exists in MCF-7 and it mainly locates the subpopulation of CD44^＋CD24^-cells,CD44^＋CD24^＋ cell possibly is breast cancer progenitor cell.

关 键 词：干细胞 乳腺癌 自我更新 动物模型 

分 类 号：R737.9[医药卫生—肿瘤]