bFGF和IGF-Ⅰ在生长期山羊颅骨缝牵张成骨中的时空表达及意义  

Temporospatial Expression of bFGF and IGF-Ⅰ in Growing Goats with Cranial Suture Distraction Osteogenesis

作　　者：刘人恺[1] 张奇峰[1] 马晓晴[1] 程敏[1] 邹淑娟[1] 

机构地区：[1]四川大学华西口腔医院正畸科,成都610041

出　　处：《四川大学学报（医学版）》2008年第4期605-608,共4页Journal of Sichuan University(Medical Sciences)

基　　金：国家自然科学基金(批准号30772454);四川省科技厅攻关课题(编号2006Z09-013)资助

摘　　要：目的观察生长期山羊颅骨冠状缝受牵张力作用后碱性成纤维细胞因子(bFGF)与胰岛素样生长因子-Ⅰ(IGF-Ⅰ)的时空表达。方法选用12只生长期山羊,用自制牵张器按0.4mm/d的速率扩张颅骨冠状逢。在牵张结束后第0、2和4周分别处死4只动物,取骨缝标本作组织学和免疫组化检测。另取2只未进行缝牵张的动物作为对照。结果bFGF与IGF-Ⅰ在牵张骨缝组织中均呈高水平表达。bFGF主要在成纤维样细胞与成骨细胞中表达,IGF-Ⅰ阳性信号主要定位于新生骨小梁边缘的成骨细胞和埋入新骨基质中的骨细胞,在骨缝扩张的0～2周这两种生长因子的表达最强。结论张应力可刺激颅骨缝细胞分泌bFGF与IGF-Ⅰ,这两种骨生长因子在骨缝牵张成骨过程中可能起着重要的调控作用。Objective To investigate the expression patterns of bFGF and IGF-Ⅰ in the growing goats with distracted cranial suture.Methods Coronal suture distraction was performed to 12 growing goats.The suture was expanded using a custom-made distractor with a rate of 0.4 mm/day for 8 days.Four goats were killed at 0,2,and 4 weeks after the completion of suture distraction osteogenesis,respectively.The expanded sutures were harvested and processed for immunohistochemistry analysis of bFGF and IGF-Ⅰ.Two goats without suture distraction were also examined as controls.Results The coronal sutures were expanded successfully.At 0 and 2 weeks after the completion of suture distraction,collagen fiber bundles were strengthened and aligned in the direction of the distraction.Strong expressions of bFGF and IGF-Ⅰ were detected in the distracted sutures.Expressions of bFGF appeared in the fibroblast-like cells and the osteoblasts cells.Positive signal of IGF-Ⅰ was mainly localized to the osteoblasts and the newly formed osteocytes.The strongest expressions of bFGF and IGF-Ⅰ were found 0-2 weeks after the completion of the suture distraction.Conclusion Distraction stimulates the production of bFGF and IGF-Ⅰ,which may contribute to the formation and remodeling of new bones.

关 键 词：骨缝牵张 碱性成纤维细胞因子 胰岛素样生长因子-Ⅰ 时空表达 

分 类 号：R782[医药卫生—口腔医学]