幽门弹簧插入配合高盐热糊灌胃复制大鼠慢性萎缩性胃炎癌前病变模型形态学观察及早期细胞凋亡分析  

Observation of pathologic picture and analysis of early apoptosis of precancerous lesion of CAG rats replicated by methods of phlorus spring insertion and hot paste

作　　者：张玉禄[1] 李军祥[1] 鲁香凤[2] 戴欣[2] 朱陵群[3] 尹军祥[2] 孙海芸[2] 

机构地区：[1]北京中医药大学东方医院,北京100078 [2]北京中医药大学东直门医院,北京100700 [3]北京中医药大学中医内科学教育部重点实验室,北京100700

出　　处：《中华中医药杂志》2008年第8期693-696,共4页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国家自然科学基金资助项目(No.30572388)

摘　　要：目的:研究幽门弹簧插入配合高盐热糊灌胃法复制慢性萎缩性胃炎(CAG)癌前病变大鼠模型形态学及早期细胞凋亡特征。方法:采用幽门弹簧插入配合高盐热糊灌胃法复制CAG癌前病变模型,用双标记流式细胞术分析CAG癌前病变大鼠细胞凋亡的特征。结果:光镜观察表明CAG癌前病变模型复制成功,CAG癌前病变模型组大鼠早期凋亡率明显高于正常组(P<0.01)。结论:幽门弹簧插入配合高盐热糊灌胃法成功复制CAG癌前病变大鼠模型,细胞凋亡增强可能是CAG癌前病变的发病机制之一。Objective： To study the feature of the pathologic picture and early apoptosis of precancerous lesion of chronic atrophic gastritis （CAG） rats replicated by methods of phlorus spring insertion and hot paste. Methods： Rats models were established by methods of phloras spring insertion and hot paste, quantify the feature of the early apoptosis of precancerous lesion of chronic atrophic gastritis rats with annexin V FITC/PI double labeled flow cytometry. Results： Pathologic picture show the rats model of precancerous lesion of CAG is convincing .The ratio of the early apoptosis of the model control group increased more significantly than that of normal group （P〈0.01）. Conclusions： It is convincing to replicate the rat model of precancerous lesion of CAG by methods of phlorus spring insertion and hot paste. Increase in the apoptosis may be one of the etiopathogenesis of precancerous lesion of CAG.

关 键 词：萎缩性胃炎 癌前病变 病理形态 流式细胞术 膜联蛋白V 大鼠 

分 类 号：R573.3[医药卫生—消化系统] R-332[医药卫生—内科学] R735.2[医药卫生—临床医学]