金针茹原生质体外源DNA转化  

Transformation of Foreign DNA into Protoplasts from Flammulina Velutipes

作　　者：成亚利[1] 朱宝成[1] 李亮亮 李庆余 

机构地区：[1]河北大学生物系

出　　处：《农业生物技术学报》1997年第4期404-408,共5页Journal of Agricultural Biotechnology

摘　　要：利用电击法将金针茹（Ｆｌａｍｍｕｌｉｎａｖｅｌｕｔｉｐｅｓ）金杂１９菌株的染色体ＤＮＡ导入金针菇０８８菌株的单核菌丝原生质体中，根据转化的双核菌丝具“锁状联合”这一形态学特征筛选出了转化菌株。转化率为（７．３８～１２．５）×１０－５，相对转化率为１４．０％～１８．９％。转化菌株的酯酶同工酶酶谱中出现了亲株没有的新酶带，说明遗传物质发生了变异。出菇实验表明，转化菌株的菌丝生长速度快于金针菇０８８菌株，现蕾期提前７天左右，菇形较好，生物学效率达９１．７％。In this paper,the total chromosome DNA of Flammulina velutipes strain 19 were transferred into protoplasts of monocaryon mycelia of Flammulina velutipes strain 088 by electroporation.The transformants were obtained by the clamp connection of dicaryon mycelia.The transformation frequency was(7.38～12.5)×10-5,the relative transformation frequency was 14.0%～18.9%.Analysing the esterase isozyme,we found the new non-parental bands appeared in the pattern of transformants,and it indicated that the genetical material had variated.In the phase of forming fruit body,the growth rate of the mycelia of the transformants was faster and the forming fruit body period was earlier than Flammulina velutipes strain 088,and the shape of fruit body was also better.The biological efficiency was 91.7%.

关 键 词：金针菇 电击法 原生质体 转化 外源DNA 

分 类 号：S646.190.3[农业科学—蔬菜学]