AQP1在骨巨细胞瘤中的表达和意义  

Expression and Distribution of Aquaporin 1 in Giant Cell Tumor of Bone and Its Significance

作　　者：刘彦宾[1] 吕智[1] 郭贵明[1] 

机构地区：[1]山西医科大学第二医院骨科,山西太原030001

出　　处：《实用骨科杂志》2008年第7期403-405,共3页Journal of Practical Orthopaedics

基　　金：山西医科大学第二医院博士基金(山西省教育厅2006214)

摘　　要：目的研究水通道蛋白1(aquaporin 1,AQP1)在骨巨细胞瘤中的分布及在骨巨细胞瘤组和对照组中的表达差别,并确定AQP1在骨巨细胞瘤各级中的表达差别,从而确定AQP1在骨巨细胞瘤中的作用。方法对42例骨巨细胞瘤标本和24例对照组标本,采用免疫组织化学方法,镜下观察AQP1在组织中的分布,应用自动图像分析检测AQP1在两组中表达情况。结果a)本免疫组化染色显示:AQP1表达于所有标本的肿瘤微血管和小血管内皮细胞上;AQP1表达于大多数骨巨细胞瘤的肿瘤细胞上,AQP1在对照组中无表达或表达很微弱;b)自动图像分析系统所得平均光密度在两组中差别显著(P<0.01),AQP1在骨巨细胞瘤中高表达;c)AQP1的表达在骨巨细胞瘤级和骨巨细胞瘤级差别显著(P<0.01),而AQP1的表达在骨巨细胞瘤其他各级之间无差别(P>0.05)。结论大多数骨巨细胞瘤的肿瘤细胞高表达AQP1的原因不明;AQP1在骨巨细胞瘤中高表达,预示其在骨巨细胞瘤的生物学行为中的作用;AQP1的表达在骨巨细胞瘤级和骨巨细胞瘤级差别显著,提示AQP1可能有区分骨巨细胞瘤级和骨巨细胞瘤级的作用。Objective To study expression level and distribution of aquaporin 1（AQP1）in giant cell tumor of bone （GCT） group and control group,and to detect the expression difference of AQP1 in GCT s grade,then try to find out the significance of AQP1 in GCT. Method AQP 1 was detected by SP immunohistochemical method. The distribution of AQP1 in tissues was observed in high-scope,and the expression level of AQP 1 was detected by auto-image analysis. Results a）This study showed that AQP 1 protein was strongly expressed in the endothelium of microvessels and small vessels in all cases;AQP 1 protein is mainly localized in the most GCT＇s rumor-cells, b）The Mean Density （MD）by auto-image analysis in GCT was also significantly higher than control groups （P〈0.01）,the data indicates that the expression level of AQP 1 in GCT was higher than that in control groups, c）The expression level in grade Ⅰ and grade Ⅲ of GCT was significantly different （P〈0.01）,but the expression level in other grades was not different （P〉0. 05）. Conclusion The AQP 1 expression level in GCT was remarkably stronger ,signify the role of AQP 1 in GCT＇s biological behaviour,moreover the expression level in grade Ⅰ and grade Ⅲ of GCT was significantly different explains AQP 1 may have the role in differenting grade Ⅰ and grade Ⅲ of GCT.

关 键 词：骨巨细胞瘤 水通道蛋白1 免疫组化 

分 类 号：R738.1[医药卫生—肿瘤]