干扰素(IFN-r)基因多态性和蛋白表达水平与卵巢癌的相关性研究  

Study on plasma IFN-r expression levels and IFN-r polymorphism in patients with susceptibility to ovarian cancer in Chinese Han population.

作　　者：何秀萍[1] 王丽丽[1] 赵辉[1] 吴迪[1] 汤海蒂[1] 曹峰林[1] 

机构地区：[1]哈尔滨医科大学第一临床医学院哈医大干细胞与生殖医学研究所,哈尔滨150001

出　　处：《中国优生与遗传杂志》2008年第7期15-16,38,共3页Chinese Journal of Birth Health & Heredity

摘　　要：目的探讨北方地区汉族人干扰素(IFN-r)基因单核苷酸多态性(SNP)和IFN-r血中表达水平与中国北方汉族人卵巢癌的关系。方法采用序列特异性引物聚合酶链反应(PCR-SSP)技术,检测33例卵巢癌患者组和90例正常对照组IFN-r基因多态性。结果结果表明卵巢癌组IFN-r(874T)等位基因频率(36.36%)显著高于对照组(10.0%),P<0.05;而卵巢癌组IFN-r血中含量(1.760±0.108)显著高于对照组(0.721±0.065),P<0.05,两组之间比较差异有显著性意义,提示该等位基因频率增高和血清IFN-r的蛋白表达与卵巢癌相关。结论我国北方地区汉族人IFN-r基因874T位点的多态性和血清IFN-r的蛋白表达水平显著升高在卵巢癌的疾病病程中有重要作用,IFN-r启动子基因874T可能是卵巢癌发病的易感性基因之一。Objective ： To study the relationship between the levels of Interferon （ IFN - r） , IFN - r gene polymorphism and ovar- ian cancer in Heilongjiang. Methods ： The polymorphisms of IFN - r gene were analyzed by sequence specific primers polymerase chain reaction （PER- SSP） methods in 33 patients with ovary cancer and 90 healthy controls. Results： Level of IFN -r in study group （ 1. 760 ± 0. 108 ） μg/L was significantly higher than the control group （0. 721 ± 0. 065 ） μg/L, P 〈 0. 05 ; Frequencies of IFN - r 874T genotype in 36. 36% of 33 patients and 10. 0% of 90 controls were significantly higher than those in healthy controls （ P 〈 0. 05 ）. Conclusion ： The level of plasma IFN - r and IFN - r 874T Polymorphism increases in patients. The differences between them have statistical significance ; Our results indicate that the change of genic polymor phism of cytokine is correlated with ovarian cancer. The predisposing IFN - r （ ＋ 874T） genetic locus may be allelomorphic gene of ovarian cancer. IFN - r genotype and T allele were significantly related to patients. IFN - r genotype can have some function susceptibility to with ovarian cancer.

关 键 词：卵巢癌 干扰素 基因多态性 

分 类 号：R737.31[医药卫生—肿瘤]