RNAi沉默VEGF表达对大肠癌细胞肝转移的影响  

Effects of RNA interference vascular endothelial growth factor expression on liver metastasis of colon cancer

作　　者：王崇强[1] 范钰[2] 徐永中[3] 庞利群[4] 

机构地区：[1]江苏大学附属医院消化科,江苏镇江212001 [2]江苏大学附属人民医院肿瘤研究所,江苏镇江212002 [3]江苏大学附属医院检验科,江苏镇江212001 [4]江苏大学附属医院普外科,江苏镇江212001

出　　处：《江苏大学学报（医学版）》2008年第4期291-294,共4页Journal of Jiangsu University：Medicine Edition

基　　金：中国博士后基金资助项目(SH2006019);镇江市科技计划资助项目(SH2006019)

摘　　要：目的:研究血管内皮生长因子(vascular endothelial growth factor,VEGF)对大肠癌肝转移的影响。方法:根据VEGF基因mRNA设计合成小干扰RNA,采用荧光实时定量PCR检测VEGF mRNA水平,分别以软琼脂集落培养试验和Boyden模型观察癌细胞锚着不依赖性增殖和癌细胞侵袭能力。以脾切除法建立大肠癌肝转移模型,以不同方法处理的癌细胞接种模型,观察对大肠癌细胞肝转移的影响。结果:转染组VEGF基因mRNA水平明显降低,且呈浓度和时间依赖性。经VEGF siRNA转染处理的癌细胞软琼脂集落形成数和穿膜细胞数明显减少,且呈浓度依赖性(P<0.05,P<0.05)。体内试验发现,对照组10只全部出现大肠癌肝转移灶,而siRNA组仅仅1只出现肝转移,差异具有显著统计学意义(P<0.01)。结论:VEGF基因在大肠癌肝转移中起着重要的作用;以RNAi技术沉默VEGF基因表达,可抑制大肠癌细胞肝转移。Objective ： To study the effects of vascular endothelial growth factor（VEGF） on liver metastasis of colon cancer. Methods ： After human colon cancer cell line LS174T were transfected by VEGF small interfering RNA（ siRNA）, the mRNA level was determined by real time RT-PCR. The anchorage-independent growth was examined using clone formation in soft agar, and invasion ability was evaluated by hoyden chamber model. Thirty nude mice model of colorectal cancer live rmetastasis was established by splenectomy. Results： VEGF siRNA can inhibit anchorage-independent growth and invasion ability in vitro, and reduce liver metastasis of colon cancer in vivo. Conclusion. VEGF siRNA can inhibit liver metastasis of colon cancer.

关 键 词：肠肿瘤 肝转移 血管内皮生长因子 RNA干扰 

分 类 号：R735.3[医药卫生—肿瘤]