高雄山虫草无性型-细脚拟青霉原生质体制备及其再生的初步研究  

Preparation of protoplast from Paecilomyces tenuipes,the anamorph of Cordyceps takaomontana

作　　者：蒲顺昌[1] 李春如[1] 汪小艳[1] 黄勃[1] 樊美珍[1] 李增智[1] 

机构地区：[1]安徽农业大学微生物防治省重点实验室,合肥230036

出　　处：《安徽农业大学学报》2008年第3期421-425,共5页Journal of Anhui Agricultural University

基　　金：国家自然科学基金面上项目(30570004);安徽省自然科学基金项目(070411011);安徽高校省级自然科学研究重点项目(TD200708)共同资助

摘　　要：对细脚拟青霉RCEF3456菌株原生质体的形成和制备有影响的多个因素,如菌丝生长时间、酶种类、酶浓度以及酶解时间等进行研究。结果表明,得到制备原生质体的最佳产量(8.0×107个.mL-1)的方法是培养18 h的菌丝、1%溶壁酶+1%Cellulase"Onozuka"R-10+1%蜗牛酶、酶解2.5 h,而且,酶解过程都是在30℃条件下进行的;同时,对原生质体的形成以及再生过程进行显微观察,再生率为1.2%。The optimum condition for protoplast preparation of Paecilomyces tenuipes RCEF3456 was investigated by studying some factors, such as mycelium age, kinds of euzymes and its concentration and enzymolysis time.The results showed that protoplasts were obtained for higher production （ 8.0×10^7/mL） under the conditions as follows：the hyphae cultured for 18 h, digested by the compound enzyme solution consisting of 1% Lywallzyme, 1% Cellulase ＂Onozuka＂ R-10 and 1% snailase for 2.5 h at 30℃. Meanwhile, formation and regeneration of protoplasts were observed,and regeneration rate was 1.2%.

关 键 词：细脚拟青霉 原生质体 酶解时间 原生质体产量 原生质体再生 

分 类 号：S476.12[农业科学—农业昆虫与害虫防治]