大肠癌Survivin、Caspase-3及NF-κBp65的实验研究  

A study of Survivin、Caspase-3 and NF-κBp65 in colorectal carcinoma

作　　者：吕小平[1] 文芬[1] 詹灵凌[1] 姜海行[1] 唐国都[1] 

机构地区：[1]广西医科大学第一附属医院消化内科,南宁市530021

出　　处：《广西医学》2008年第7期954-957,F0002,共5页Guangxi Medical Journal

基　　金：广西自然科学基金资助课题(桂科基0342033);广西科学研究与技术开发计划项目(桂科攻0592007-1Z)

摘　　要：目的研究凋亡相关蛋白Survivin、Caspase-3和NF-κBp65在正常大肠黏膜、大肠腺瘤及大肠腺癌组织中的表达,探讨它们在大肠癌癌变过程中的作用与其临床病理特征的关系。方法采用免疫组化SABC法分别检测大肠腺癌、大肠腺瘤及正常大肠黏膜组织中的Survivin、Caspase-3和NF-κBp65的表达。结果Survivin、Caspase-3和NF-κBp65分别在大肠腺癌、大肠腺及正常大肠黏膜的表达差异有统计学意义(P<0.013)。Survivin、Caspase-3和NF-κBp65的阳性表达率在大肠腺癌中不同组织学分级间差异均有统计学意义(P<0.05),组织分化程度越低,Survivin和NF-κBp65的表达强度越强,则Caspase-3的表达强度则越弱。Survivin与Caspase-3在大肠腺癌中的表达呈负相关(P<0.05)。结论大肠腺癌中Survivin和NF-κBp65表达上调,提示Survivin和NF-κBp65的高表达共同参与了大肠腺癌的发生。Survivin和NF-κBp65随大肠腺癌组织分化程度降低而表达上调,提示Survivin和NF-κBp65可在一定程度上反映大肠腺癌的恶性程度。Survivin可通过抑制Caspase-3的表达,促进肿瘤的发生。Objective To study the expression and significance of Survivin,Caspase-3 and NF-κBp65 in tumorigenesis of colorectal Methods The expression of Survivin, Caspase-3 and NF-κBp65 were detected by in colorectal adenocareinoma, colorectal adenoma and normal colorectal mucesa SABC immunohiatechenfical assay. Results The expression of Survivin and NF-κBp65 increased in colorectal adenecarcinoma and had a significant difference from normal colorectal mucosa and coloreetal adenoma （P 〈0.013）. The expression of Caspase-3 decreased in colorectal adenocareinoma and had a significant difference from normal colorectal mucesa and colorectal adenoma （P 〈0.013）. The expression of Survivin and NF-κBp65 were higher in moderate and poor differentiation degree than those in high differentiation degree in coloreetal adenecarcinoma（P 〈0.05）. The expression of Caspase-3 was lower in moderate and poor differentiation degree than those in high differentiation degree in coloreetal adenecareinoma（P 〈0.05）. The expression of Survivin was significant negative correlate with Caspase-3 in colorectal adenecareinoma （P 〈 0. 05 ）. Conclusion The expression of Survivin and NF-κBp65 increases in colorectal adenecareinoma. Survivin and NF-κBp65 closely correlates to ttanorigenesis of coloreetal adenocareinoma. The Survivin and NF-κBp65 increases while the eolorectal adenecarcinoma differentiation degree＇s depressed,it indicated that the high expression of Survivin and NF-κBp65 may be the reflection of colorectal adenecaminoma＇s malignant feature. The expression of Survivin in carcinoma is negative related to the expression of Caspase-3. It confered that Survivin could restrain the activation of Caspase-3 ,and promote the development.

关 键 词：大肠腺癌 大肠腺瘤 SURVIVIN NF-ΚBP65 CASPASE-3 免疫组化SABC法 

分 类 号：R735.34[医药卫生—肿瘤]