裸鼠皮下人肝癌种植瘤模型的建立  

Establishment of the nude mice model transplanted with human liver cancer

作　　者：易石坚 李兰兰 钟德玝[2] 杨竹林[2] 段伦喜[2] 

机构地区：[1]深圳市福永人民医院,广东深圳518103 [2]中南大学湘雅二医院肝胆外科,湖南长沙410011

出　　处：《中国医药导报》2008年第21期27-28,共2页China Medical Herald

基　　金：湖南省科技计划项目;项目编号:05SK3023

摘　　要：目的:建立裸鼠皮下人肝癌种植瘤模型。方法:BLBA/c裸鼠颈背部皮下注射人肝癌SMMC-7721细胞悬液5×106个/(0.2ml·只),观察肿瘤生长情况,10周处死。瘤组织作病理及电镜检查;取种植裸鼠周围血作甲胎蛋白(AFP)的检测,随机抽出10只未行处理的BLBA/c雄性裸鼠作对照;用免疫组化法检测肿瘤微血管密度(MVD),取正常非瘤组织作对照。结果:种植裸鼠周围血甲胎蛋白(AFP)为(528.35±12.48)ng/ml,对照组为(135.37±9.27)ng/ml,两组比较,有显著性差异(P<0.05);种植裸鼠平均每中倍视野中的微血管数为(32.45±2.38)个。对照组为(9.16±1.21)个,两组比较,有显著性差异(P<0.05)。该模型具有类似人原发性肝癌的形态学特征。结论:成功建立了人肝癌裸鼠皮下种植瘤模型。Objectlve：To construct nude mice sub-skin model transplanted with human liver cancer.Methods： 5×10^6/0.2 ml of human liver cancer cell strain SMMC-7721 suspension were injected into sub-skin in the neck of nude mice. The growth of tumor was observed and the mice were executed after 10 weeks.Pathologic examination, detection of AFP in serum by radiation immunization, electronmicroscope observation and detection of MVD by immunohistochemistry were used to observe the growth condition of tumor.Results：The model constructed had the same morphological character compared with the human liver cancer.Conclusion：The nude mice sub-skin model transplanted with human liver cancer is constructed successfully.

关 键 词：肝癌 种植 裸鼠模型 

分 类 号：R735[医药卫生—肿瘤]