饲料蛋白对翘嘴鲌生长和内分泌激素的影响  

EFFECT OF DIETARY PROTEIN ON GROWTH AND HORMONE OF CULTER ALBURNUS JUVENILES

作　　者：王桂芹[1] 周洪琪[2] 陈建明[3] 叶金云[3] 赵朝阳[2] 周辉[2] 闫大伟[2] 潘茜[3] 王友慧[3] 

机构地区：[1]吉林农业大学动物科技学院,长春130118 [2]上海水产大学生命学院,上海200090 [3]浙江省淡水水产研究所,湖州313001

出　　处：《水生生物学报》2008年第4期544-550,共7页Acta Hydrobiologica Sinica

基　　金：浙江省重大科技攻关项目(2002C12016);浙江省湖州市科技重点项目(2003GN04)资助

摘　　要：选择健康的翘嘴鲌(Culter alburnus)为试验鱼,以红鱼粉为蛋白源,配制5个蛋白水平的等能、等必需氨基酸(EAA)平衡关联度的半精制饲料,又以豆粕替代鱼粉,大豆蛋白分别替代不同水平的鱼粉蛋白,配制5个EAA关联度的等蛋白、等能的半精制饲料,探讨饲料蛋白对鱼类生长和内分泌激素等的影响。结果表明:饲料蛋白水平对翘嘴鲌的特定增重率(SGR)具有显著影响(p<0.05),40.89%饲料蛋白组的SGR显著高于31.04%、35.51%饲料蛋白组(p<0.05),但与46.62%和50.33%饲料蛋白组没有显著性差异(p>0.05)。血清T3水平与饲料蛋白水平正相关(p<0.05)。血清GH水平与饲料蛋白水平和生长负相关(p<0.05),血清IGF-I水平与饲料蛋白水平和生长正相关(p<0.05)。适宜蛋白水平通过提高翘嘴鲌血清T3和IGF-I的水平来调控生长。当大豆蛋白分别替代54.0%的鱼粉蛋白时,翘嘴鲌的SGR与对照组差异显著(p<0.05)。翘嘴鲌血清T3与饲料大豆蛋白对鱼粉蛋白的替代量负相关(p<0.05)。40.5%和54.0%替代组的血清GH显著高于对照组(p<0.05),且与饲料中豆粕对鱼粉替代水平正相关(p<0.01),与生长负相关(p<0.05)。血清IGF-I与饲料中大豆蛋白对鱼粉蛋白替代量负相关(p<0.05),与生长正相关(p<0.05)。大豆蛋白的替代亦通过对内分泌激素的影响来调控生长。A series of growth experiments were conducted by using brown fishmeal as the protein source in formulated diets. Culter alburnus with initial weight（ （ 12.84 ± 0.60） g wet weight） were fed with 5 isoenergetic（20MJ/g gross energy） , the same relativity of essential amino acid balance experimental semi-purified diets, containing different protein levels （31.04% ,35.51% ,40.89% ,46.62% and 50. 33% dry matter） in 5% increments,five isonitrigenous （40% protein） and isoenergetic （20 MJ/g gross energy） and different relativity of essential amino acid balance experimental semi-purified diets with soybean protein replacing 0.0% , 13.5% ,27： 0% ,40.5% and 54.0% of fish meal protein,were formulated. After 8-week trial, hormone in serum were measured using radioimmunoassay to examine the effect of dietary protein level and replacement of fish meal by soybean cake on growth and triiodothyronine （ T3 ）, thyroxine （ T4 ）, growth hormone （GH） and insulin-like growth factor-I （IGF-I） of Culter alburnus juveniles. The results showed that SGR was significantly influenced by dietary protein levels （p 〈 0.05 ）. SGR of the fish fed the 40.89% protein diets was significantly higher than that of 31.04% and 35.51% protein diets （p 〈0.05） ,but there was not significantly different from that of 46.62% and 50.33% protein diets （p 〉 0.05）. Serum T3 was a positive correlation with dietary protein level （p 〈 0.05 ） ,which increased with increasing of dietary protein level. Serum GH decreased significantly with increasing dietary protein level,which showed a negative correlation with fish growth. Serum IGF-I showed a positive correlation with dietary protein level and growth （p 〈 0.05 ）. Optimum dietary protein level can regulate growth by increasing serum T3 and IGF-I level. When the replacement level for fish meal protein with soybean protein was 13.5% ,27% and 40.5% ,respectively, there were no significant difference in specific growth rate compared with the control g

关 键 词：翘嘴鲌 饲料蛋白水平 大豆蛋白 甲状腺激素 生长激素 类胰岛素生长因子-I 

分 类 号：S965.1[农业科学—水产养殖]