拟南芥VHA-c3启动子的GUS基因融合表达  

Localization of Arabidopsis VHA-c3 Promoter with GUS-Fusion

作　　者：郭荣起[1] 苏杰[1] 王福慧[1] 徐萍[2] 伊莉佳[1] 姜树原[3] 王瑞刚[1] 

机构地区：[1]内蒙古农业大学生物工程学院,呼和浩特010018 [2]华东师范大学生命科学学院,上海200062 [3]包头医学院生物医学中心,包头014010

出　　处：《中国生物工程杂志》2008年第7期58-62,共5页China Biotechnology

基　　金：国家自然科学基金(30460018);内蒙古自然科学基金(200711020509)资助项目

摘　　要：VHA-c3基因是拟南芥液泡H+-ATPasec亚基的5个同源基因之一,已有研究结果表明其与植物抗非生物胁迫有关。在克隆不同长度VHA-c3基因上游调控序列的基础上,利用GUS报告基因,研究了VHA-c3基因的植物组织及器官定位。结果表明:VHA-c3起始密码子上游772bp的序列内存在着VHA-c3基因的基本启动子元件,可指导基因组成型表达在拟南芥的叶片、表皮毛、叶柄、根、雄蕊、柱头和萼片;在VHA-c3起始密码子ATG上游2812bp～2234bp片段和1496bp～772bp片段中各存在一个负调控元件,在2234bp～1496bp片段中存在一个正调控元件,它们的存在可控制基因在气孔中的表达。VHA-c3 gene is one of the five homologous genes which belong to the subunits of vacuole H^＋ - ATPase in Arabidopsis thaliana. Former reports indicated that it is related with resistance to abiotie stress in plants. Different fragments of the upstream regulation sequences of Arabidopsis VHA-c3 gene were cloned, and fused with GUS reporter gene, to study the localization of VHA-c3 gene. The essential element for the basal activity of VHA-c3 is located within the -722bp fragments upstream of the translation start eodon of VHA-c3, it can guide genes＂ constitutive expression in leaf blade, triehome, petiole, root, stamen, stigma and sepal in Arabidopsis; And there is two negative regulatory elements located in -2812bp to -2234bp and -1496bp to -772bp respectively, and an up-regulating eis-element from -2234bp to -1496bp fragment of VHA-c3 gene, which may control the gene＇s expression in guard cell.

关 键 词：拟南芥 V-ATPASE VHA-c3基因 启动子 

分 类 号：Q786[生物学—分子生物学]