高压氧治疗对家兔实验性大脑中动脉闭塞后梗死灶周围巢蛋白表达的影响  

Effect of hyperbaric oxygen treatment on expression of nestin in a model of right middle cerebral artery occlusion in rabbits

作　　者：潘杰香[1] 李飞[1] 储卫华[1] 冯华[1] 

机构地区：[1]第三军医大学西南医院神经外科,全军神经系统疾病微创诊治专科中心,重庆400038

出　　处：《中华神经医学杂志》2008年第7期657-660,共4页Chinese Journal of Neuromedicine

基　　金：国家“十一五”科技支撑计划（2006BAl01A13）;全军医学科学技术研究“十一五”课题（062037.06MB241）

摘　　要：目的探讨高压氧（HBO）治疗对兔大脑中动脉闭塞（MCAO）模型梗死灶周围巢蛋白（nestin）表达的影响。方法制备兔右侧MCA0模型，将动物分为3组，即MCAO＋HBO治疗组（30只）：MCA0后立即行HB0治疗，每天1次，连续20d；MCAO对照组（30只）：MCA0后不行HB0治疗；假手术组（10只1：仅开颅不闭塞血管。分别于MCA0后10d、20d观察其行为学、梗死体积变化．并用免疫组化法检测距梗死灶中心3mm内皮层脑组织内nestin的表达。结果MCA0后，MCAO＋HBO治疗组行为学评分高于MCAO对照组，差异有统计学意义（P〈0．05）；MCAO＋HBO治疗组脑梗死体积小于MCA0对照组，差异有统计学意义（P〈0．051；MACO后10d、20dMCA0＋HB0治疗组nestin免疫阳性细胞数（15．88±1．2、20．03±1．6）高于MCA0对照组（6．63±1．6、6．82±0．8），差异有统计学意义（P〈0．05）。结论早期、连续20dHBO治疗对兔MCAO后的神经行为学、梗死体积有明显的治疗效果，并促进兔MCAO后梗死灶周围脑组织内nestin的表达。Objective To evaluate the effect of hyperbaric oxygen （HBO） treatment on the expression of nestin in a model of right middle cerebral artery occlusion （MCAO） in rabbits. Methods Seventy healthy rabbits were divided into 3 groups： the rabbits in MCAO＋HBO group（n=30） were treated with HBO immediately after MCAO, once a day, consecutively for 20 d; the rabbits in MCAO group （n=30） were not treated with any therapy after MCAO; the control group （n=10） received the same operation as that of MCAO group except MCAO. Rabbit behaviors and volumes of infarction were observed, and the expression of nestin was measured by immunocytochemistry 10 and 20 d after MCAO. Results Behaviors＇scores and volumes of infarction were better in MCAO＋HBO group than the MCAO group （P〈0.05）. 10 d after MCAO, the number of nestin-immunoreactive cells of MCAO＋HBO group and MCAO group was respectively 15.88±1.2 and 6.63±1.6; 20 d after MCAO, the number in MCAO＋HBO group and MCAO group was respectively 20.03±1.6 and 6.82±0.8. The number of nestin-immunoreactive cells in MCAO＋HBO group was significantly higher compared with that of MCAO group （P〈0.05）. Conclusion Early applications of HBO and repeated administration of HBO for 20 d can evidently improve the behaviors and infarct size and elevate the expression of nestin in rabbit models of MCAO.

关 键 词：高压氧治疗 大脑中动脉闭塞 巢蛋白 

分 类 号：R743.3[医药卫生—神经病学与精神病学]