诱导供体特异性前房相关免疫偏离对高危角膜移植排斥反应的影响  

Effect of donor-specific anterior chamber-associated immune deviation on the rejection of corneal allograft in high-risk eyes

作　　者：李文静[1,2] 高晓唯 任兵 郭继华 王绍飞[1,2] 

机构地区：[1]石河子大学医学院,中国新疆维吾尔自治区石河子市832002 [2]解放军第四七四医院眼科中心,中国新疆维吾尔自治区乌鲁木齐市830011

出　　处：《国际眼科杂志》2008年第5期903-906,共4页International Eye Science

基　　金：军队医药科研基金项目资助(NoLXH-2005027)~~

摘　　要：目的:探讨诱导供体特异性的前房相关免疫偏离(anterior chamber-associated immune deviation,ACAID)对高危角膜移植排斥反应的影响。方法:采用新西兰白兔眼角膜建立碱烧伤眼模型,实验动物随机分为4组,A:正常角膜常规角膜移植组(正常对照组);B:碱烧伤常规角膜移植组(碱烧伤对照组);C:正常角膜诱导ACAID的角膜移植组(正常诱导组)。D:碱烧伤后诱导ACAID的角膜移植组(碱烧伤诱导组)。B,D组进行左眼碱烧伤,烧伤后1mo进行角膜移植。C,D组在角膜移植前2wk于右眼前房注入可溶性抗原以预先诱导供体特异性ACAID。A,B组右眼前房注等量平衡盐溶液。术后记录植片存活时间;对角膜新生血管(corneal neovascularization,CNV)生长情况进行评分;记录移植排斥指数(rejection index,RI);角膜固定、包埋后制作切片行HE染色。结果:A、C组角膜植片长期存活,碱烧伤对照组植片平均存活25.13±0.64d,碱烧伤诱导组角膜植片平均存活时间为38.25±1.28d。与碱烧伤对照组相比,碱烧伤诱导ACAID组角膜植片平均存活时间显著延长,两组植片存活时间的差异具有统计学意义(P=0.00)。结论:诱导供体特异性ACAID可以延长碱烧伤高危眼角膜植片的存活时间。AIM： To investigate the effect of donor-specific anterior chamber-associated immune deviation （ACAID） on the rejection of corneal allograft in high-risk eyes. METHODS： Corneal injuries were induced by alkaline burn in New Zealand white rabbits to establish ocular models. These rabbits were randomly divided into four groups： A： normal cornea of keratoplasty group; B： alkali burned cornea keratoplasty group; C： normal cornea induced by ACAID keratoplasty group; and D： alkali burned cornea induced by ACAID keratoplasty group. The left cornea of group B and D were burned by alkali, and 1 month later orthotopic keratoplasty was performed. At 2 weeks before keratoplasty, donor-specific ACAID was induced by injecting soluble antigen into recipient＇s anterior chamber in group C and D, whereas balanced saline solution was injected into the right eye anterior chamber of group A and B. Graft mean survival time, neovascularization and rejection index were determined. The cornea was performed HE staining. RESULTS.. The grafts of groups A and C survived in long term. The graft mean survival time of group B was 25.13 ±0.64 days. The graft mean survival time of group D was 38.25± 1.28 days. Corneal allograft survival of group D was enhanced （P=0.00） compared with that of group B. CONCLUSION： Induction of donor-specific ACAID can effectively prolong corneal allograft survival time in alkali burned high-risk eyes.

关 键 词：免疫偏离 穿透性角膜移植术 碱烧伤 排斥反应 

分 类 号：R779.65[医药卫生—眼科] R770.3[医药卫生—临床医学]