AG对大鼠慢性高眼压视网膜Caspase-9表达的影响(英文)  

Expression of caspase-9 affected by AG on retina of rats with chronic IOP elevation

作　　者：聂庆珠[1] 沙倩[1] 王英爽[1] 归东梅[1] 刘致力[1] 高殿文[1] 

机构地区：[1]中国医科大学附属盛京医院眼科,中国辽宁省沈阳市110004

出　　处：《国际眼科杂志》2008年第7期1299-1303,共5页International Eye Science

摘　　要：目的:研究慢性高眼压过程中大鼠视网膜Caspase-9表达的变化及应用AG对其表达的影响,探讨AG可能存在的对慢性高眼压视网膜的保护作用。方法:应用免疫组化、RT-PCR和Western blot的方法观察应用AG及未应用AG的慢性高眼压大鼠视网膜在慢性高眼压后不同时间点的形态学变化及Caspase-9表达的变化。结果:与正常对照组相比,随着高眼压时间的延长视网膜逐渐出现可察觉的形态学变化,于高眼压的第21d视网膜变薄,节细胞数量减少;在此过程中,Caspase-9表达增多,与形态学变化相一致。而应用AG者其形态学变化和Caspase-9表达变化较小。结论:凋亡相关基因Caspase-9在慢性高眼压视网膜损伤过程中发挥了作用,AG通过下调其表达对视网膜起保护作用。AIM： To study caspase-9 expression on rat retina in the process of chronic elevation of lOP and the changes with the application of amino guanidine （AG）, thus to investigate potential protective function of AG to rat retina with chronic elevation of IOP. METHODS： Immunohistochemistry, RT-PCR and Western blot were used to observe retinal morphology and expression of caspase-9 at different time points of rat with chronic lOP elevation, both affected or not affected by the application of AG. RESULTS： Compared with control group, as time passed retina of experimental group gradually had detectable morphological changes. On 21st day of chronic lOP elevation, retinas became thinner and the quantity of retinal ganglion cells （RGCs） decreased; caspase-9 expression increased, consistent with the morphological changes. The group using AG presented relatively smaller morphology changes and less expression of caspase-9. CONCLUSION ： Apoptosis-related gene caspase-9 played a part in the process of chronic IOP elevation ; AG protects retina by down-regulating expression of caspase-9.

关 键 词：视网膜 慢性高眼压 凋亡 氨基胍(AG) 半胱天冬酶-9(Caspase-9) 

分 类 号：R775[医药卫生—眼科] R774.1[医药卫生—临床医学]