马铃薯微型薯切块离体再生的研究  

Regeneration of Minituber Disc Explants in Potato

作　　者：王晓杰[1] 乔利仙[1] 张敏凤[1] 李翠芹 郭宝太[1] 

机构地区：[1]青岛农业大学生命科学学院,山东青岛266109 [2]莱阳市种子公司

出　　处：《青岛农业大学学报（自然科学版）》2008年第2期120-122,共3页Journal of Qingdao Agricultural University(Natural Science)

摘　　要：在MS+2mg/L IAA+1mg/L 6-BA+4mg/L ZT+6mg/L KT的培养基上,鲁引1号、夏波蒂、早大白、大西洋四个马铃薯品种的脱毒微型薯切块都可直接再生出不定芽,其中鲁引1号的诱导率为63.3%,其它3个品种的诱导率均在90.0%以上。不定芽及其形成的小苗在MS+0.15%活性炭培养基上可顺利生根,形成完整的植株。Minituber disc explants of four potato varieties were inoculated into the medium of MS ＋ 2mg/L IAA ＋ lmg/L 6 -BA ＋4mg/L ZT ＋6mg/L KT. Adventitious buds were directly induced from the explants. Adventitious bud differentiation rate of Luyin - 1 was 63. 3% and the rates were more than 90.0% in other three varieties of Shepody, Zaodabai and Atlantic. Optimal medium of adventitious bud induction was successfully selected. Adventitious buds or shoots produced roots in MS ＋0.15% C medium, and regeneration potato plants were formed.

关 键 词：马铃薯 微型薯切块 不定芽 再生植株 

分 类 号：Q813.12[生物学—生物工程]