毛头鬼伞(Coprinus comatus)中一种抗病毒蛋白y3特性和氨基酸序列分析(英文)  

Characterization and Amino Acid Sequence of y3,an Antiviral Protein from Mushroom Coprinus comatus

作　　者：吴丽萍[1] 吴祖建[1] 林董[1] 方芳[1] 林奇英[1] 谢联辉[1] 

机构地区：[1]福建农林大学植物病毒研究所

出　　处：《中国生物化学与分子生物学报》2008年第7期597-603,共7页Chinese Journal of Biochemistry and Molecular Biology

基　　金：福建省科技厅重大专项(No2006NZ0002-2)资助;江西省自然科学基金(No2007GQN2001)资助项目~~

摘　　要：食用菌中含有多种抗病毒蛋白,可用于植物保护.利用离子交换层析技术和凝胶层析技术,从食用菌毛头鬼伞中提取到抗植物病毒蛋白y3.实验结果表明,y3是一种糖蛋白,利用Western杂交方法可以在发酵菌丝体和子实体中同时检测到,说明可能是组成型表达.根据其N端氨基酸序列,使用RACE-PCR克隆技术,获得了蛋白的氨基酸序列和部分cDNA序列.浓度为2.0μg/ml时,蛋白y3对烟草花叶病毒(TMV,20μg/ml)侵染心叶烟的抑制率为50%,实验同时表明,y3还可抑制病毒在寄主普通烟Nicotiana tabacum var.K326中的复制.Many proteins from mushrooms can be used as potential antiviral agents and in plant protection. An antiviral protein, designated as y3, was isolated from fruiting bodies of the fungus Coprinus comatus by fastflow ion-exchange column chromatography coupled with high-resolution molecular sieve chromatography. This glycoprotein was detected in both fruiting body and mycelium by Western blot analysis. According to its Nterminal sequence, an amino acid sequence and a partial cDNA sequence of the y3 gene were obtained. Protein y3 at 2.0 μg/ml achieved 50.0% inhibition of tobacco mosaic virus （TMV, 20 μg/ml） lesions in Nicotiana glutinosa leaves. It also inhibited multiplication of TMV in Nicotiana tabacum var. K326.

关 键 词：食用菌 毛头鬼伞 蛋白 抗病毒活性 克隆 

分 类 号：Q78[生物学—分子生物学]