S100A2基因及其蛋白在食管癌中的表达及其临床意义  

Expression of S100A2 mRNA and protein and its clinical significance in esophageal cancer

作　　者：尹玉[1] 张洪福[1] 

机构地区：[1]安徽医科大学病理解剖学教研室,安徽省合肥市230032

出　　处：《世界华人消化杂志》2008年第19期2119-2124,共6页World Chinese Journal of Digestology

摘　　要：目的：观察S100A2 mRNA及其蛋白在食管鳞状细胞癌组织中的表达及其与食管癌临床病理特征之间的关系，并探讨其在食管癌发生、发展中的作用及临床应用价值． 方法：收集40例手术切除的食管鳞状细胞癌标本，同时取40例手术切缘食管正常黏膜组织．应用免疫组织化学S-P法检测其中S100A2蛋白的表达，原位分子杂交技术检测S100A2 mRNA的表达． 结果：S100A2 mRNA及S100A2蛋白在食管鳞状细胞癌组织中阳性表达率均明显低于正常食管黏膜（77．5％vs100％，72．5％vs100％，均P〈0．01）；其中高、中、低分化三组间差异均有显著性（均P〈0．05），并且高、中分化组S100A2 mRNA的阳性表达率均明显高于低分化组（分别为933％vs85．7％，86．7％vs85．7％，均P〈0．05）;无淋巴结转移组S100A2 mRNA阳性表达率均明显高于有淋巴结转移组（分别为92％vs53．3％，92％vs40％，均P〈0．01）．食管鳞状细胞癌组织中S100A2 mRNA和S100A2蛋白的表达呈明显正相关（r=0．607，P〈0．001）. 结论：S100A2在食管癌的发生发展中具有重要作用，并可能作为判断食管癌生物学行为的重要参考指标．AIM： To investigate the expression of S100A2 mRNA and its protein in esophageal squamous cell cancer（ESCC） and their relationship with clinicopathologic features, and explore their roles in carcinogenesis and progression of esophageal cancer and to evaluate their clinicopathologic significance. METHODS： Immunohistochemical staining （S-P method） was performed to detect S100A2 protein expression in 40 ESCC samples and 40 samples from normal esophageal mucosa. In situ hybridization （ISH） was performed to detect the expression of S100A2 mRNA. RESULTS： The positive rates of S100A2 mRNA and S100A2 protein in ESCC samples were both markedly lower than that in normal mucosa （77.5% vs 100%, 72.5% vs 100%, both P 〈 0.01）. The differences were both significant among the well differentiated, the moderately differentiated and the lowly differentiated groups （all P 〈 0.05）. The expression levels of S100A2 mRNA and S100A2 protein were significantly higher in well and moderately differentiated groups than that in lowly differentiated group （93.3% vs 85.7%, 86.7% vs 85.7%, both P 〈 0.05）. There was significant difference between the lymph node-positive group and the lymph node-negative group （92% vs 53.3%, 92% vs 40%, both P 〈 0.01）. A positive correlation was found between the expression of S100A2 mRNA and S100A2 protein （r = 0.607, P 〈 0.001）. CONCLUSION： S100A2 plays an important role in ESCC carcinogenesis. S100A2 might be an important reference biomarker for ESCC biological behavior.

关 键 词：食管肿瘤 S100A2 原位分子杂交 免疫组织化学 

分 类 号：R735.1[医药卫生—肿瘤]