散发性结直肠癌中hMSH2与PTEN的表达及临床意义  

Expression of hMSH2 and PTEN protein in sporadic colorectal carcinoma and their clinical significances

作　　者：宋伟庆[1] 范占彬[1] 王烨[1] 袁虎方[1] 杨彦林[1] 王利伟[1] 韩彩丽[2] 

机构地区：[1]河北医科大学第二医院胃肠外科,河北省石家庄市050000 [2]河北医科大学病理教研室,河北省石家庄市050017

出　　处：《世界华人消化杂志》2008年第19期2125-2130,共6页World Chinese Journal of Digestology

基　　金：河北省自然科学基金资助项目;No.C2008001116~~

摘　　要：目的:研究错配修复蛋白hMSH2与PTEN在散发性结直肠癌的表达变化及两者之间的关系,以进一步探讨其临床意义.方法:采用SP免疫组织化学两步法对42例散发性结直肠癌、相应近端癌旁组织(距癌组织3cm)和远端癌旁组织(距癌组织>10cm)及15例正常结直肠组织进行hMSH2与PTEN蛋白表达检测.同时Western blot法检测42例散发性结直肠癌、相应远端对照组织hMSH2与PTEN蛋白表达.分析散发性结直肠癌发病机制及hMSH2与PTEN蛋白表达与临床病理之间的关系.结果:结直肠癌组织中,hMSH2与PTEN蛋白失表达率(阴性率)均低于正常结直肠组织及近端癌旁组织、远端癌旁组织(χ2hM S H2=7.967,χ2PTEN=11.67,均P<0.05).PTEN蛋白表达与肿瘤分化程度呈正相关(rs=0.727,P<0.05),与Dukes分期、侵润深度、淋巴结转移、肝转移均呈负相关(rs=-0.727,-0.718,-0.718,-0.535,均P<0.05).hMSH2蛋白表达与上述临床病理特征未见明显相关.h M S H2与P T E N蛋白表达在散发性结直肠癌中呈正相关(rs=0.679,P<0.05).与远端对照组织相比42例胃癌组织中hMSH2蛋白低表达率为59.52%(25/42),PTEN低表达率为45.24%(19/42).结论:PTEN表达与其临床病理特征相关;在散发性结直肠癌发生、发展过程中错配修复蛋白hMSH2表达缺失伴有PTEN蛋白表达下调.AIM： To investigate expression alteration of human mut-s homologue 2 （hMSH2） and PTEN in sporadic colorectal carcinoma （SCC） and thereby to elucidate their relationship and clinical significance. METHODS： Expression levels of hMSH2 and PTEN were detected using immunohistochemistry in 42 cases of SCC, its corresponding adjacent tissues （3 cm away from cancerous tissues）, distal tissues （from cancer 〉 10 cm） and 15 cases of normal colorectal tissues. The expression levels of hMSH2 and PTEN protein were analyzed using Western blot in 42 cases of SCC and its corresponding distal controls. RESULTS： Both negative expression of hMSH2 and PTEN were higher in SCC than in adjacent tissues, distal tissues or normal colorectal tissues （χ^2hMSH2 = 7.967, χ^2PTEN = 11.667, both P 〈 0.05）. PTEN protein expression was positively correlated with tumor differentiation （Гs = 0.727 P 〈 0.05） and negatively correlated with Dukes stage, invasive depth, lymph node metastasis and liver metastasis（Гs = -0.727, -0.718, -0.718, -0.535, all P 〈 0.05）. No significant correlation was observed between hMSH2 protein expression and SCC pathological features. There was a positive correlation between of the hMSH2 and PTEN protein in SCC （Гs = 0.679, P 〈 0.05）. Compared with the corresponding distal tissues, there were 25 cases of （59.52% 25/42） lower expression of hMSH2 protein and 19 cases （45.24% 19/42）of lower expression of PTEN protein in SCC. CONCLUSION： PTEN protein expression is correlated with clinic and pathological features. The expression deletion of mismatch repair protein hMSH2 accompanied by down-regulated PTEN protein expression occur in SCC development.

关 键 词：散发性结直肠癌 HMSH2 PTEN 免疫组织化学 免疫印迹法 

分 类 号：R735.3[医药卫生—肿瘤]