IL-10对TGF-β_1诱导的大鼠肝星状细胞CTGF表达的影响  

Effect of interleukin-10 on TGF-β_1-induced CTGF expression in hepatic stellate cells

作　　者：杨悦杰[1] 黄芬[1] 胡静[1] 马力[1] 李智伟[1] 

机构地区：[1]中国医科大学附属盛京医院感染科,辽宁省沈阳市110004

出　　处：《世界华人消化杂志》2008年第19期2154-2157,共4页World Chinese Journal of Digestology

摘　　要：目的:探讨IL-10对大鼠肝星状细胞(hepatic stellate cells,HSC)表达CTGF的影响及其抗纤维化的可能机制.方法:体外培养大鼠肝星状细胞系rHSC-99,分别用不同浓度的IL-10、TGF-β1干预和两者共同干预48h后,采用半定量RT-PCR法检测各组肝星状细胞中CTGF mRNA表达水平.结果:TGF-β1单独干预HSC,CTGF mRNA表达水平与对照组比较均明显增强(P<0.05);不同浓度的IL-10单独干预HSC,各目的基因表达与对照组比较无明显统计学意义;不同浓度的IL-10与TGFβ1共同干预HSC,CTGF mRNA表达水平与TGF-β1组比较均明显降低(P<0.05).结论:IL-10可显著抑制由TGF-β1诱导的CTGF mRNA的表达,这可能是其抗纤维化的机制之一.AIM： To investigate the effect of interleukin-10 （IL-10） on the expression of connective tissue growth factor （CTGF） in hepatic stellate cells （HSC） and its possible antifibrogenic mechanism. METHODS： Hepatic stellate cells （rHSC-99） cultured in vitro was exposed to various concentrations of IL-10 and/or TGF-β1 for 48 h and then expression level of CTGF mRNA was measured by semiuantitative reverse-transcription polymerase chain reaction （RT-PCR）. RESULTS： The expression level of CTGF mRNA, compared with control group, markedly increased when HSC was only exposed to TGF-β1 （P 〈 0.05）; when HSC was only exposed to various concentrations of IL-10, target gene expression showed no significance; the expression level of CTGF mRNA markedly decreased when HSC was exposed to TGF-β1 in combina-tion with different concentrations of IL-IO （P 〈 0.05）. CONCLUSION： IL-10 remarkably inhibits TGF- β1-induced CTGF mRNA expression, which is thought to be one possible antifibrogenic mechanism.

关 键 词：白介素-10 转化生长因子 肝星状细胞 结缔组织生长因子 

分 类 号：R575.2[医药卫生—消化系统] R692.6[医药卫生—内科学]