PML-RARα-hIL-2 DNA疫苗诱导BALB／c小鼠的细胞和体液免疫应答  

Immune response induced by reconstructed PML-RARα-hIL-2 plasmids in BALB/c mice

作　　者：杨力建[1] 岑东芝[1] 周羽竝[2] 胡刚[1] 陈少华[1] 李扬秋[1] 

机构地区：[1]暨南大学医学院血液病研究所,广州510632 [2]暨南大学医学院生化教研室,广州510632

出　　处：《癌症进展》2008年第4期367-370,381,共5页Oncology Progress

基　　金：广东省科技计划项目(2005B50301016);广州市科技计划项目(2003J1-I0011;2005Z1-E4015);中德生物技术合作项目(CHN02/319);血液病学国务院侨办重点学科建设基金

摘　　要：目的分析PML-RARα融合基因疫苗诱导小鼠特异体液和细胞免疫应答情况。方法用成功构建的重组质粒(pIRES-PML-RARα-hIL-2)免疫小鼠后,分别利用ELISA法检测小鼠血清中INF-γ生成情况;LDH释放法检测小鼠脾细胞特异性CTL细胞针对APL细胞株NB4的杀伤率;用间接免疫荧光法检测血清中抗PML-RARα抗体。结果免疫后第7周时,小鼠血清中INF-γ含量、抗PML-RARα抗体及脾脏CTL细胞针对NB4的杀伤率均高于对照组。结论所构建的pIRES-PML-RARα-hIL-2重组质粒可诱导小鼠产生了特异性体液和细胞免疫应答。Objective To evaluate the in vivo immune effect induced by PML - RARα vaccines in mice. Methods After immunization with reconstructed pIRES-PML-RARα-hIL-2, the specific cytotoxicity of spleen cells from thevaccinated BALB/C mice was detected by LDH release assay; interferon-γ secretion was measured by ELISA; antibodies against human PML-RARα were detected by indirect immunofluorescene analysis. Results Time dependent antibody production and an increase in INF-γ were be detected in vaccinated mice serum. Specific cytotoxicities on NB4 cells were significantly higher than those in control groups. Conclusion and cellular immune The recombinant plRES-PML-RARα hIL-2 plasmids can induce specific humoral responses in BALB/c micie in vivo.

关 键 词：PML-RARΑ HIL-2 急性早幼粒细胞白血病 DNA疫苗 细胞毒活性 抗体 

分 类 号：R733.71[医药卫生—肿瘤]