PML-RARα-hIL-2 DNA疫苗免疫BALB／c小鼠后胸腺初始T细胞水平变化  

The change of thymic nave T cells in BALB/c mice after immunization with PML-RARα-hIL-2 DNA vaccine

作　　者：周羽竝[1] 岑东芝[2] 杨力建[2] 陈少华[2] 胡刚[2] 李扬秋[2] 

机构地区：[1]暨南大学医学院生化教研室,广州510632 [2]暨南大学医学院血液病研究所,广州510632

出　　处：《癌症进展》2008年第4期371-375,共5页Oncology Progress

基　　金：广东省科技计划项目(2005B50301016);广州市科技计划项目(2003J1-I0011;2005Z1-E4015);中德生物技术合作项目(CHN02/319);血液病学国务院侨办重点学科建设基金

摘　　要：目的了解PML-RARα-hIL-2基因疫苗免疫小鼠后胸腺初始(na(?)ve)T细胞变化情况。方法利用实时定量PCR检测小鼠胸腺组织DNA中T细胞受体删除DNA环(TRECs)的水平,从而评价小鼠胸腺中na(?)ve T细胞含量。结果利用PML- RARα-hIL-2 DNA疫苗免疫小鼠后胸腺中所含的TRECs拷贝数明显高于单个基因疫苗PML-RARα或hIL-2组及对照组。结论所构建的PML-RARα-hIL-2双表达质粒可诱导小鼠胸腺产生na(?)ve T细胞数量增加,可能与产生更强的细胞免疫应答有关。To analyze the change of naive T cells level in mice thymus after immunization with PML- RARα-hIL-2 DNA vaccine. Methods T-cell receptor rearrangement excision circles （TRECs） in DNA from mice thymic cells were detected by real-time quantitative RT-PCR （TaqMan） , and then the content of naive T cells was evaluated. Results TRECs level form PML-RARα-hIL-2 vaccine group was signifi- cantly higher than those from PML-RARα, hIL-2 vaccine and control groups. Conclusion The recombinant PML-RARα-hIL-2 DNA vaccine can induce the increase of naive T cells in mice thymus, which may be due to stronger cellular immune response.

关 键 词：PML-RARΑ HIL-2 TRECS DNA疫苗 

分 类 号：R733.71[医药卫生—肿瘤]