高效液相色谱法测定山茱萸与五味子配伍前后马钱苷、没食子酸及五味子醇甲的含量  

Determination of Loganin,Gallic Acid and Schisandrin Contents before and after the Combination of Fructus Corni with Fructus Schisandrae Chinensis

作　　者：周莉莉[1] 吴国光[1] 刘志强[2] 刘淑莹[2] 

机构地区：[1]中国矿业大学化工学院,江苏徐州221008 [2]中国科学院长春应用化学研究所.质谱中心,吉林长春130022

出　　处：《时珍国医国药》2008年第4期782-783,共2页Lishizhen Medicine and Materia Medica Research

基　　金：国家自然科学基金(No.20173057);中国科学院知识创新工程重要方向项目(No.KGCX2-SW-213-06)

摘　　要：目的考察山茱萸与五味子配伍前后两药主要组分的含量变化规律。方法利用高效液相色谱(HPLC)方法,比较了配伍前后单煎液、合煎液和单煎合并液中两药主要组分马钱苷、没食子酸和五味子醇甲的含量变化。结果在配伍比例较低(≤4:4)时,五味子促进了山茱萸中马钱苷的溶出。合煎液中的没食子酸、五味子醇甲组分的百分含量随着五味子配伍比例的增大呈线性减小,且均低于单煎液。单煎合并液中这3种组分的含量均高于同比例合煎液。结论山茱萸五味子配伍对两药主要组分溶出有较大影响,该影响主要在煎煮过程中发生。Objective To investigate the content variation of main chemical constituents before and after combination of Fructus Corni with Fructus Schisandrae Chinensis. Methods Fructus Corni and Fructus Sehisandrae Chinensis were decocted with water separately and the separate aqueous extract were combined partly. Different proportions of Fructus Corni with Fruetus Schisandrae Chinensis at different proportions were decocted together. Then the contents of loganin, gallic acid and schisandrin were determined by high performance liquid chromatography （HPLC） . Results Fructus Sehisandrae Chinensis could promote the dissolve of Loganin at low combined proportions. The contents of gallic acid and schisandrin were declined linearly with the increase of Fructus Schisandrae Chinensis in combination, and they were all lower than those in the separate aqueous extract. The contents of the three constituents in incorporated aqueous extract of Fructus Corni and Fructus Schisandrae Chinensis were all overtop the same proportion together - decoction. Conclusion The great influence of the combination of Fructus Corni with Fructus Schisandrae Chinensis on the contents of main chemical constituents was mainly carried on in the decoction process which provides a certain scientific basis for the reasonable Chinese herbal medicine.

关 键 词：山茱萸 五味子 配伍 马钱苷 五味子醇甲 没食子酸 高效液相色谱法 

分 类 号：R284.2[医药卫生—中药学] R289.1[医药卫生—中医学]