CXCR4/SDF-1α对宫颈癌HeLa细胞定向迁移及增殖的影响  

Effect of CXCR4/SDF-1α in Derected Migration and Proliferation of HeLa Cells

作　　者：张建平[1] 吕卫国[2] 谢幸[2] 

机构地区：[1]苏州大学附属一院妇产科,215006 [2]浙江大学附属妇产科医院妇瘤科

出　　处：《肿瘤防治研究》2008年第7期495-498,共4页Cancer Research on Prevention and Treatment

基　　金：浙江省教育厅科研项目基金资助(20040217)

摘　　要：目的了解CXCR4在HeLa细胞的表达情况，并借助细胞培养评价CXCR4／SDF-1α对HeLa细胞定向迁移及增殖的影响。方法CXCR4mAb免疫染色HeLa细胞。用Transwell侵袭转移模型评价HeLa细胞的迁移情况，其中，上室中加入预先用（或不用）CXCR4单抗预孵育的HeLa细胞，下室中加入含0～100ng／ml SDF-1α的培养基。为评价CXCR4、SDF-1α对HeLa细胞增殖的影响，将HeLa细胞接种于有（无）SDD-1α和（或）CXCR4的低血清环境72h。结果CXCR4在所有HeLa细胞上均有表达。HeLa细胞能顺SDF-1α浓度差定向迁移，且这一作用可被CXCR4mAb拮抗。SDF-1α能促进HeLa细胞在低血清环境中增殖。结论CXCR4／SDF-1α参与了HeLa细胞定向迁移的过程并影响其增殖．Objective To investigate the expression of CXCR4 in HeLa cells, and evaluate the effect of CXCR4/SDF-1α in directed migration and proliferation of HeLa cells. Methods HeLa cells were immunohistochemically stained with mouse anti-CXCR4 monoclonal antibody （mAb）. In vitro invasion of HeLa cells was evaluated using Transwell Permeable Supports,in which HeLa cells with/without CXCR4 mAb pre-incubation were seeded in the upper chambers, medium containing 0-100 ng/ml of SDF-1α was added to the lower compartments. For evaluating the effect of CXCR4/SDF-1α on proliferation of cervical cancer cells, HeLa cells were cultured for 72 hours exposed to SDF-1α with and without CXCR4 mAb. Results CXCR4 was expressed on all HeLa cells. SDF-1α induced the directed migration of HeLa cells with a concentration-depended model, which was inhibited by using CXCR4 mAb. SDF-1α also stimulated the proliferation of HeLa cells mediated by CXCR4. Conclusion CXCR4/ SDF-1α participate in the directed migration and proliferation of HeLa cells.

关 键 词：HELA细胞株 定向迁移 增殖 CXCR4 SDF-1Α 

分 类 号：R737.33[医药卫生—肿瘤]