重组人促红细胞生成素的CE和CE-MS检测方法研究进展  

Recent Advance in the CE and CE-MS Detection of Recombinant Human Erythropoietin in Doping Control

作　　者：庞楠楠[1] 杨霞[1] 廖一平[1] 刘虎威[1] 

机构地区：[1]北京大学化学与分子工程学院,北京100871

出　　处：《生命科学仪器》2008年第7期14-19,共6页Life Science Instruments

基　　金：国家自然科学基金资助项目(No.20635001)

摘　　要：促红细胞生成素(erythropoietin,EPO)是调控红血球细胞量的糖蛋白激素,近年来被滥用在一些耐力性比赛项目中。由于EPO在尿样或血样中的浓度低,代谢快以及结构复杂多变给检测带来了很大的难度。该文从毛细管电泳(capillary electrophoresis,CE)和毛细管电泳-质谱(CE-mass spectrometry,CE-MS)角度综述了近几年来兴奋剂重组人促红细胞生成素(recombinant human EPO,rhEPO)检测的研究进展。Erythropoietin is a glycoprotein hormone that stimulates erythropoiesis and maintains the red blood cell mass at an optimum level. In the recent years, recombinant human EPO （rhEPO） has been often misused in endurance competition sports. The detection of rhEPO involves a series of analytical challenges due to its relatively short half-life, very low concentration in blood or urine and the high complexity of the mixture of glycoforms. The present review summarized the separation and the detection of rhEPO by capillary electrophoresis （CE） as well as by CE on-line coupled with mass spectrometry （MS） and we also discuss the further development of these techniques.

关 键 词：重组人促红细胞生成素 兴奋剂 毛细管电泳 质谱 综述 

分 类 号：O657.8[理学—分析化学] TQ467[理学—化学]