中华眼镜蛇膜毒素和碘-131协同免疫导向对体外鼻咽癌细胞的抑制作用  

Inhibitory effects of immunotargeting of Chinese cobra cytotoxin and iodine-131 against nasopharyngeal carcinoma cells in vitro

作　　者：赵永胜[1] 杨惠玲[2] 刘长征[3] 

机构地区：[1]南方医科大学附属深圳医院/深圳市第二人民医院核医学科,广东深圳518035 [2]中山大学医学院病理生理学教研室,广东广州510089 [3]中山大学医学院实验核医学教研室,广东广州510089

出　　处：《南方医科大学学报》2008年第7期1235-1236,1239,共3页Journal of Southern Medical University

基　　金：国家自然科学基金(39370781)~~

摘　　要：目的为探索鼻咽癌新的特异性治疗方法,制备了中华眼镜蛇膜毒素(CT)和碘-131(131I)与抗鼻咽癌单克隆抗体BAC5的偶联物,观察其对体外鼻咽癌细胞CNE2的抑制作用。方法采用氯胺T法将131I标记于BAC5,Iodogen法将125I标记于CT,用异型双功能交联剂SPDP偶联BAC5和125I-CT,并用噻唑蓝法观察抑瘤效应。结果125I-CT-BAC5的IC50为9.17×10-8mol/L,131I-BAC5的IC50为5.83×108Bq/L,联合用药后抑制率明显提高。结论125I-CT-BAC5和131I-BAC5对体外培养的CNE2细胞均有抑制作用,二者联合应用则抑制作用更为明显。Objective To explore a new specific therapy of nasopharyngeal carcinoma （NPC） using an anti-nasopharyngeal carcinoma （NPC） monoclonal antibody BAC5 conjugate with Chinese cobra （CT） and iodine-131 （131Ⅰ）. Methods BAC5 was labeled with 131Ⅰ by chloramine-T method, CT was labeled with 125Ⅰ using iodogen method, and BAC5 and 125Ⅰ-CT were conjugated by SPDP method. The inhibitory effect of the conjugate on cultured human NPC CNE2 cells was observed using MTT assay. Results The IC50 of 125Ⅰ-CT-BAC5 conjugate was 9.17×10^-8 mol/L, and that of 131Ⅰ-BAC5 was 5.83×10^8 Bq/L, and their combined administration showed obvious inhibitory effect on the NPC cells. Conclusion Both 125Ⅰ-CT-BAC5 and 131Ⅰ-BAC5 have obvious inhibition effects against NPC cells, but their combined use shows stronger effects.

关 键 词：中华眼镜蛇膜毒素 碘-131 鼻咽癌 抗体 单克隆 

分 类 号：R739.63[医药卫生—肿瘤]