乳腺癌HER2／neu手性多肽疫苗的实验研究  

Experimental study of HER2/neu chiral polypeptide vaccine of breast cancer

作　　者：王芳[1] 王毅[1] 宿晓云[1] 周余来[1] 侯立中[1] 才子斌[2] 

机构地区：[1]吉林大学药学院,吉林长春130021 [2]吉林大学第一临床医院

出　　处：《中国老年学杂志》2008年第14期1367-1369,共3页Chinese Journal of Gerontology

基　　金：国家自然科学基金资助(30600743)

摘　　要：目的研究具有局部手性的HER-2/neu多肽疫苗对细胞毒性T细胞(CTLs)的诱导作用。方法用固相法合成HER-2/neu手性肽疫苗并进行纯化,建立小鼠的乳腺癌模型,用上述手性肽做疫苗、重组人粒细胞-巨噬细胞集落刺激因子(GM-CSF)为佐剂,对小鼠进行免疫,分别应用MTT法、ELISPOT法、颗粒酶释放法测定T细胞的增殖,T细胞分泌IFN-γ,CTL对肿瘤细胞的杀伤活性。结果合成的手性HER-2/neu可明显的促进T细胞增殖,促进T细胞分泌IFN-γ及诱导CTL杀伤乳腺癌细胞。结论手性HER-2/neu多肽疫苗可在小鼠体内诱导特异细胞免疫,增加对乳腺癌细胞的杀伤作用。Objective To study the induction of HER-2/neu specific CTLs in mice by partial chiral peptide vaccine. Methods The HER-2/neu chiral peptide was synthesized using solid phase peptide synthesis method and purified. Breast cancer model of BALB/c mice was constructed, HER-2/neu chiral peptides were used as vaccine, GM-CSF was used as adjuvant to activate cell immunity. MTT, ELISPOT and relapse method of granule enzyme were adopted respectively to test the proliferation of the T cells , IFN-γ secretion by T cells and the ability of CTLs to kill the cancer cells. Results Cell immunity was stimulated by chiral HER-2/neu peptide vaccine including T cells proliferation, the incremental secretion of IFN-γ by T cells, activation of CTLs to kill breast cancer cell. Conclusions The chiral HER-2/neu peptide vaccine could induce HER-2/neu specific CTLs in mice, activate cell immunity, enhance the ability to kill breast cancer cell.

关 键 词：乳腺癌 手性 疫苗 HER-2/NEU 细胞毒性T细胞(CTLs) 

分 类 号：R73[医药卫生—肿瘤] R9[医药卫生—临床医学]