高效液相色谱法测定食品中禁用色素苏丹红Ⅰ-Ⅳ和对位红  

Determination of the Banned Pigment SudanⅠ-Ⅳ Red and Para Red in food Stuffs by HPLC

作　　者：胡冬生 

机构地区：[1]南海出入境检验检疫局,佛山528200

出　　处：《理化检验（化学分册）》2008年第7期675-677,共3页Physical Testing and Chemical Analysis(Part B:Chemical Analysis)

摘　　要：采用高效液相色谱法测定食品中禁用色素苏丹红Ⅰ-Ⅳ和对位红。以乙腈为提取剂,超声波提取样品中苏丹红Ⅰ-Ⅳ和对位红。以ZORBAX SB-C_(18)柱(4.6 mm×150 mm,5μm)为分离柱,乙酸(1+999)溶液和乙腈为流动相进行梯度洗脱,流速为1 mL·min^(-1),检测波长为485 nm的条件下进行检测。苏丹红、对位红的回收率为90%～98%,相对标准偏差小于5%,苏丹红和对位红的检出限均为0.01μg·g^(-1),苏丹红和对位红的质量浓度在0.2～2.0 mg·L^(-1)范围内呈线性。An accurate and sensitive method for the determination of the banned pigments sudan red and para red in foodstuffs by HPLC was proposed. Sudan red and para red in foodstuffs were extracted in a ultrasonic washing machine with acetonitrile. ZORBAX SB-C18 clounm （4. 6 min× 150 mm, 5 μm） was used as the stationary phase, and mixtures of dil. HOAc（1 ＋ 999） and acetonitrile in different ratios were used as mobile phase in the gradient elution, at a flow-rate of 1 mL · min^-1. Photometric deterctions were made at 485 nm. Recoveries of sudan red and para red were in the range of 90M-98M and RSD＇s （n=6） found were less than 5%. Detection limits of the method was found to be 0. 01μg · g^-1. Linear relationship was kept in the range Of 0. 2-2. 0 mg· L^-1.

关 键 词：高效液相色谱法 食品 苏丹红 对位红 

分 类 号：O657.37[理学—分析化学]