预运动训练对脑梗死大鼠脑保护作用的兴奋性氨基酸递质效应  

The effect of treadmill pre-training on the brain protection in rat′s cerebral infarction with the excitatory amino acid neurotransmitter dynamic changes

作　　者：胡永善[1] 贾杰[1] 吴毅[1] 刘罡[1] 于惠贤[1] 曹志娟[2] 夏春梅[3] 

机构地区：[1]复旦大学附属华山医院康复医学科,复旦大学神经生物学国家重点实验室,复旦大学上海医学院康复与运动医学系,上海200040 [2]复旦大学药学院药物分析所 [3]复旦大学医学院生理病理学系

出　　处：《中国康复医学杂志》2008年第7期589-593,共5页Chinese Journal of Rehabilitation Medicine

基　　金：国家自然科学基金(30772300);中国博士后基金(20070420597)

摘　　要：目的:动态观察预运动训练对大鼠脑梗死后纹状体处兴奋性氨基酸水平变化的影响,探讨预运动对缺血脑损伤的保护作用机制。方法:将SD大鼠随机分为5组,每组实验用时均为4周:运动1周组(运动训练1周,在第4周实施),运动2周组(运动训练2周,在后2周实施),运动4周组(运动训练4周),假手术组和缺血组。各组大鼠在4周后,立体定位在脑内纹状体(尾状核)留置微透析管,进行大脑中动脉缺血术,采用在体微透析技术收集大鼠缺血前、缺血期间(40,80和120min)和再灌注后(40,80,120,160,200和240min)的脑细胞外液。测定纹状体(尾状核)脑区兴奋性氨基酸谷氨酸(Glu)和天门冬氨酸(Asp)含量的变化。同时测量计算缺血再灌注24h的脑梗死体积。结果:2周和4周的预运动训练可以显著下调因缺血而过度释放的Glu和Asp水平(P<0.01)。缺血再灌注24h的不同组的脑梗死体积变化差异有显著意义(P<0.05)。结论:至少2周的预运动训练对随后发生的脑缺血及再灌注期间,纹状体(尾状核)脑区内兴奋性氨基酸递质Glu和Asp的过度释放有一定程度的抑制作用,这种抑制效应对早期缺血脑损伤具有一定的保护作用。Objective：To observe the dynamic effects of treadmill pre-training on excitatory amino acid changes in striatum of rats with cerebral infarction and to explore the mechanism of pretraining protection to ischemic brain injury. Method： Sprague-Dawley rats were randomly divided into 5 groups： two control groups （sham and ischemia）, 1 week group （trained in the fourth week）, 2 weeks group（trained in the last two weeks） and 4 weeks group. After four weeks, the rats were fixed on stereotactic orientation for the brain microdialysis of the striatum. Then the model of middle cerebral artery ischemia and reperfusion was made with thread occlusion in rats and microdialysis technique was used to collect extracellular fluid in each time point of preischemic, ischemic （40th min, 80th min, 120th min）, and reperfusion （40th min, 80th min, 120th min, 160th min, 200th min, 240th min） to inspect the changes of excitatory amino acid glutamate（Glu） and aspartate（Asp） content with the main reference values. At the same time infarct volumes of brains at ischemia-reperfusion 24 hours were measured. Result： Two weeks and four weeks treadmill pre-training could significantly reduce Glu and Asp excessive release due to ischemia （P〈0.01, P〈 0.01）. Infarct volume changes at ischemia-reperfusion 24 hours in different groups had a significant difference （P〈 0.05）. Conclusion： At least two weeks treadmill pre-training can in a certain degree restrain excessive release of the important excitatory amino acid neurotransmitter glutamate and aspartate in striatum in the process of subsequent ischemic-reperfusion brain injury, which may be one of the protection mechanisms for the early ischemic brain injury.

关 键 词：脑梗死 预运动 脑内微透析 兴奋性氨基酸 脑保护 

分 类 号：R743.3[医药卫生—神经病学与精神病学] R493[医药卫生—临床医学]