姜黄素治疗大鼠肝纤维化及其对肝组织Ⅰ、Ⅲ型胶原的影响  

Therapeutic effects of curcumin on hepatic fibrosis of rat and its influence on expression of collagen alphalⅠand Ⅲ in liver

作　　者：舒建昌 邓亮 叶国荣 何雅军 吕霞 杨小红 

机构地区：[1]暨南大学医学院附属广州红十字会医院,广东广州510220

出　　处：《国际内科学杂志》2008年第7期379-382,共4页International Journal of Internal Medicine

基　　金：广东省中医药局科研基金(1040147);广州市卫生局科研基金(2004A041)

摘　　要：目的观察姜黄素治疗肝纤维化的效果及其对肝组织Ⅰ、Ⅲ型胶原的影响,探讨两者间的关系。方法以四氯化碳腹腔注射的方法制作大鼠肝纤维化模型,姜黄素治疗组大鼠于第9周起分别给予姜黄素10、20、40mg/100g灌胃,设立正常对照组、溶剂对照组、模型组和丹参组,检测各组大鼠血清丙氨酸氨基转移酶(ALT)、天门冬氨酸氨基转移酶(AST)、透明质酸(HA)、层黏蛋白(LN)、Ⅲ型前胶原肽(PC-Ⅲ)含量,肝组织行HE和Masson胶原染色,光镜下观察肝脏病理学改变,并按肝纤维化半定量计分系统(SSS)进行评分。免疫组织化学染色观察肝组织Ⅰ、Ⅲ型胶原的变化,专业图像分析软件进行分析。结果与模型组比较,姜黄素治疗组大鼠肝纤维化时异常升高的ALT、AST、HA、LN、PC-Ⅲ明显降低(P<0.05);大鼠肝纤维化的病理学改变明显改善,肝纤维化评分明显降低(P<0.05),接近正常组;肝纤维化大鼠肝组织Ⅰ、Ⅲ型胶原含量明显降低,且这种效果随着姜黄素剂量的增大而增强。结论姜黄素具有治疗大鼠肝纤维化的作用;增强胶原纤维降解可能是其重要的作用机制之一。Objective To investigate the effect of curcumin on hepatic fibrosis and expression of collagen alphal Ⅰ and Ⅲ in liver of hepatic fibrosis rat, and thus to explore the relationship between them. Methods Rat hepatic fibrosis model was made with carbon tetrachloride. Cureumin of 10, 20, 40 mg per 100 g was given to rats in curcumin groups respectively from the ninth week after eaton tetrachloride was injected. Normal group, dissolvent group, model group and Salvia miltiorrhiza group were made as controls. Serum levels of alamine aminotransferase （ALT） , aspartate aminotransferase （AST） , hyaluronic acid （HA） , Laminin （LN） , pmcollagen Ⅲ （ PC- Ⅲ ） were detected. The pathological variation of hepatic tissues was detected with HE and Masson staining. Grades of hepatic fibrosis were evaluated according to the SSS system. Collagen alphal Ⅰ and Ⅲ in liver were also detected with immunohistochemical staining, and professional software was used for image analysis. Results Curcumin could obviously decrease serum level of ALT, AST, HA, LN, Pc-Ⅲ （P 〈 0. 05 ）, which were increased in fibrotic moldel group. Curcumin could obviously improve liver pathological variation of fibrotic rats. Curcumin treatment could significantly reduce the score of hepatic fibrosis （ P 〈 0. 05 ） and the expression of collagen alphal Ⅰ and Ⅲ in liver, besides, these effects of curcumin were dose-dependent. Conclusion Curcumin could lighten the degree of hepatic fibrosis and enhance degradation of collagen in liver tissue may be one of its important mechanisms.

关 键 词：姜黄素 肝纤维化 胶原纤维 病理 

分 类 号：R575.2[医药卫生—消化系统]