环孢霉素A对OVA免疫TCR转基因DO11．10小鼠CD4^＋CD25^＋T细胞的影响  

The effect of cyclosporin A on CD4^+CD25^+T cells in TCR-transgenic DO11.10 mice immunized with OVA

作　　者：王弘珺[1] 马海霞[2] 李质馨[1] 赵勇[2] 

机构地区：[1]吉林医药学院组织胚胎学教研室,吉林132013 [2]中国科学院动物研究所生物膜与膜生物工程国家重点实验室移植生物研究组,北京100101

出　　处：《中国免疫学杂志》2008年第7期588-590,共3页Chinese Journal of Immunology

基　　金：973项目(2003CB51550)

摘　　要：目的:研究免疫抑制剂CsA对处于免疫应答状态的小鼠脾脏调节性CD4+CD25+T细胞影响。方法:采用流式细胞术检测卵白蛋白(OVA)免疫的DO11.10小鼠脾脏CD4+CD25+T细胞及其中Foxp3+T细胞的变化。结果:OVA免疫后脾脏CD4+CD25+T细胞占CD4+T细胞百分数及Foxp3+T细胞占CD4+CD25+T细胞百分数均增加,显示CsA可明显减少正常及免疫应答状态下小鼠脾脏CD4+CD25+T细胞及CD4+CD25+Foxp3+T细胞百分数。结论:CsA在抑制免疫应答的同时也可能抑制了免疫耐受的诱导。Objective： To study the effect of CsA on CD4^＋CD25^＋ T cells from DOI 1 -10 mice after immunization with OVA. 1Methods： The amount of spleen CD4^＋CD25^＋ T cells and Foxp3 ＋ T cells was detected by means of flow cytometry （FCM）. Results： After immunization with OVA, the ratios of CD4^＋CD25^＋ T cells to CD4＋ T cells and Foxp3 ＋ T cells to CD4^＋CD25^＋ T cells elevated in spleen, but CsA significantly decreased the percentage of CD4^＋CD25^＋ T cells and CD4^＋CD25^＋ Foxp3 ＋ T cells under either naive state or immune reaction state. Conclusion： CsA inhibits immune responses, while it also suppresses immune tolerance.

关 键 词：CD4^+CD25^+T细胞 CSA OVA 

分 类 号：R392.1[医药卫生—免疫学]