ING4基因对人胃癌细胞SGC-7901生物行为的影响  

Effect of ING4 gene transfection on biological characteristics of human tumor cell line SGC-7901

作　　者：黄俊琼[1] 孙万邦[1] 张海峰[2] 杨吉成[2] 

机构地区：[1]遵义医学院珠海校区免疫学教研室,珠海519041 [2]苏州大学医学院细胞与分子生物学教研室,苏州215123

出　　处：《中国免疫学杂志》2008年第7期612-614,617,共4页Chinese Journal of Immunology

摘　　要：目的:研究人ING4对人胃癌细胞SGC-7901的影响,探讨其作用机制。方法:将重组腺病毒质粒pAd-ING4用PacI线性化,经脂质体转染QBI-293A细胞,获得重组病毒Ad-ING4。Ad-ING4感染人SGC-7901细胞,RT-PCR及Western blot分析ING4和p21表达,MTT法检测Ad-ING4对细胞生长的影响,激光共聚焦显微镜检测细胞凋亡。结果:Ad-ING4感染后,SGC-7901细胞中有ING4 mRNA和蛋白质表达,细胞生长受到明显抑制,与野生型SGC-7901细胞相比,p21表达水平增高,细胞凋亡率明显增高,P<0.05。结论:ING4可通过上调p21表达发挥抑制SGC-7901细胞生长、诱导细胞凋亡的作用。Objective：To investigate the effect of IGN4 gene trasfection on SGC-7901 cells, and the possible mechanism for its anti-tumor effect .Methods： Ad-ING4 was obtained by gene recombination and packaging technique in vitro. The expression of ING4 mRNA and protein were analyzed by RT-PCR and western-blot respectively. The effect of Ad-ING4 on growth of SGC-7901 cells was detected by MTT. Apoptotic ceils were detected by Laser Scan Co-focal Microscope （LSCM）. The expression of p21 in SC, C-7901 cells was analyzed by western-blot, Results： After infection with Ad-ING4, the expression of ING4 mRNA and protein were showed in SGC-7901 cells detected. The proliferation of SGC-7901 cells was inhibited significantly（ P 〈 0.05）, acompanied by increased cell apoptosis. The expression of p21 was higher than in wildtype SGC-7901 cells. Condusion：Ad-ING4 can inhibit the growth of SGC-7901 cells by up-regulating the expression of p21.

关 键 词：ING4 AD-ING4 人SGC-7901细胞 P21 

分 类 号：R73-3[医药卫生—肿瘤]