单增李斯特菌不同PCR快速检测方法比较  

Comparison of three rapid PCR methods for the detection of Listeria monocytogenes

作　　者：李盛丰[1] 赵姣[1] 钟名华[1] 夏国亮[1] 邓灵福[1] 张筱丽[1] 冯莹颖[1] 罗勤[1] 

机构地区：[1]华中师范大学生命科学学院,武汉430079

出　　处：《中国公共卫生》2008年第8期1021-1023,共3页Chinese Journal of Public Health

基　　金：国家自然科学基金项目(30500025);教育部211重点学科项目资助;华中师范大学大学生创新性实验项目

摘　　要：目的比较3种PCR快速检测方法在检测单核细胞增生李斯特菌时的特异性和灵敏度方面的差异。方法针对单核细胞增生李斯特菌的毒力基因iap和prfA基因,分别设计引物,进行单个PCR、多重PCR、套式PCR等3种方法检测。结果3种PCR方法均具有较强的特异性;套式PCR的灵敏度为102cfu/ml,高于单个PCR(103cfu/ml)和多重PCR(104cfu/ml)。结论多重PCR在特异性检测方面具有优势,而在灵敏度方面,套式PCR要明显优于单个及多重PCR方法。Objective To investigate the differences among the three fast- detect PCR methods in their specificity and sensitivity for the detection of Listeria monocytogenes （LM）. Methods Based on the sequence of LM virulence gene lap and prfA, five pairs of primers were designed for each of the three PCR. Results All the primers showed high specificity. The sensitivity of the nested- PCR got as high as 102 cfu/ml, which was more sensitive than the single- PCR （103 cfu/ml） and the multiplex - PCR（104 cfu/ml）. Conclusion Multiplex - PCR has more advantages in specificity. However, nested - PCR was more sensitive than single - PCR and multiplex - PCR obviously. Therefore, the primary health and quarantine departments should choose a suitable method according to their situations and the purpose of supervision work.

关 键 词：单核细胞增生李斯特菌 单个PCR 多重PCR 套式PCR 特异性 灵敏度 

分 类 号：R446.1[医药卫生—诊断学]