停跳液细菌内毒素检查  

Tachpleus Amebocyte Lysate Test for Bacterial Endotoxins in Cardioplegia Solution

作　　者：陈德坚[1] 

机构地区：[1]海南省人民医院,海口市570311

出　　处：《中国药房》2008年第22期1745-1747,共3页China Pharmacy

摘　　要：目的:建立停跳液的细菌内毒素检查方法。方法:根据2005年版《中国药典》(二部)附录细菌内毒素检查法进行试验。结果:停跳液稀释至8倍时对细菌内毒素的检查无干扰,Et在0.5Es～2Es范围内。结论:停跳液用灵敏度为0.06EU.mL-1的鲎试剂检查细菌内毒素是可行的。OBJECTIVE： To establish the method of assaying bacterial endotoxin in cardioplegia solution. METHODS： The tachpleus amebocyte lysate （TAL） test for bacterial endotoxin in cardioplegia solution was performed in accordance with Chinese Pharmacopoeia （2005 Edition, Volume Two） . RESULTS： The 8-fold diluents of cardioplegia solution did not interfere with the test for bacterial endotoxins, with Et = 0.5 Es-- 2 Es. CONCLUSION ： It is feasible to detect bacterial endotoxins in cardioplegia solution with TAL at a sensitivity of 0.06 EU· mL^-1.

关 键 词：停跳液 细菌内毒素 鲎试验 

分 类 号：R927.11[医药卫生—药学]