沈抚灌区石油污染土壤微生物多样性的研究  

A Study on Microbial Diversity of Petroleum Contaminated Soil in Shenfu Irrigation Area

作　　者：何晶晶[1] 李慧[2] 张颖[2] 王纯利[1] 

机构地区：[1]新疆农业大学草业与环境科学学院,乌鲁木齐830052 [2]中国科学院沈阳应用生态研究所中国科学院陆地生态过程重点实验室,沈阳110016

出　　处：《新疆农业大学学报》2008年第4期33-37,共5页Journal of Xinjiang Agricultural University

基　　金：中国科学院陆地生态过程重点实验室基金项目;国家重点基础研究发展计划项目(2004CB418505)

摘　　要：以不依赖于培养的16S rDNA-PCR-ARDRA技术系统评价了我国最大的石油污水灌区—沈抚灌区污灌对土壤细菌遗传多样性的影响,并对微生物群落中的优势菌群进行了研究。结果表明,沈抚灌区土样总石油烃(TPH)含量分布呈递减趋势,细菌基因多样性与各样点TPH含量呈显著负相关(r=-0.990,P=0.046)。优势OTUs测序结果表明,沈抚灌区土壤细菌群落中的优势菌群为变形细菌(Proteobacteria)β-亚群和衣原体属(Chlamydiae)的菌种。The effects of petroleum contamination on bacterial quantities, diversities and dominant populations in the soils were investigated in Shenfu irrigation area, the largest area irrigated by oil-containing wastewater in northeast of China. The bacterial diversities were determined by conventional colony morphology and 16S rDNA PCR-amplified ribosomal DNA restriction analysis （ARDRA）. The results showed that soil total petroleum hydrocarbon （TPH） concentration in the irrigation area presented degressive trend. The genetic diversity based on ARDRA profiles was negatively correlated with TPH concentration （r =-0.990,P =0.046）. Sequence analysis of prominent OTUs showed that βsubclass of Proteobacteria and Chlamydiae were dominant in Shenfu irrigation area.

关 键 词：石油污染 土壤 细菌多样性 ARDRA 

分 类 号：X172[环境科学与工程—环境科学]