新疆牛环形泰勒虫Tams1基因的克隆与序列分析  

Cloning and Sequence Analysis of the Bovine Theileria annulata Tams1 Gene in Xinjiang

作　　者：黄家雨[1] 马素贞[1] 苗中秋[1] 袁江玲[1] 苏贵成[1] 沈炯玉[1] 张兰江 李晓军 简子健[1] 

机构地区：[1]新疆农业大学动物医学学院,乌鲁木齐830052 [2]克拉玛依市乌尔禾乡政府,克拉玛依834012 [3]克拉玛依市乌尔禾乡兽医站,克拉玛依834012

出　　处：《新疆农业大学学报》2008年第4期69-73,共5页Journal of Xinjiang Agricultural University

基　　金：国家自然科学基金项目(30660141)

摘　　要：采用PCR技术，从新疆焦虫病疫区感染牛的全血中扩增到Tamsl基因，该基因长度为846bp，编码281个氨基酸。同源性分析表明，该基因与其他9个国际流行虫株的核苷酸同源性为93．6％～99．8％，氨基酸同源性为88．6％～99．6％。系统发育进化树分析表明，新疆分离株同印度株、毛利塔尼亚株、土耳其株、北非共和国株进化途径一致，与苏丹株和巴林株进化途径相差较远。氨基酸抗原决定簇分析发现新疆分离株编码的氨基酸有13个抗原决定簇。In order to analyze gene sequence of the bovine Theileria annulata in Xinjiang,a pair of primers was designed, Tamsl gene fragment was amplified by PCR, sequence of positive clones was showed that the gene fragment has a total length of 846 bp,which encoded 281 amine acids. The homology of the nucleotide sequence was 93.6%--99.8% ,and homology of the Amino acid sequence was 88. 6%--99.6% compared with other 9 international prevalent strains. The phylogenetic evolution tree was shown that the isolated with other 9 international prevalent strains. The phylogenetic evolution tree was shown that the isolated Xinjiang strain is closely related to those from India,Mauritania,Turkey,Tunisia,but not as well to those from Bahrain and Sudan. The analysis of antigenic determinant was shown that there were 13 antigenic determinats in the encoded strains isolated from Xinjiang Tams1.

关 键 词：新疆 牛环形泰勒虫 裂殖子表面抗原 克隆与序列分析 

分 类 号：S851.723[农业科学—预防兽医学]