藏药三味檀香散对大鼠心肌缺血-再灌注损伤的抗氧化作用  

The Antioxidative Effect of Sanwei Tanxiang Powder on Rats′ Hearts against Myocardial Ischemia and Reperfusion Injury

作　　者：寇毅英[1] 扎西多杰[1] 李永芳[1] 格日力[1] 

机构地区：[1]青海大学医学院,青海西宁810001

出　　处：《中药材》2008年第7期1013-1015,共3页Journal of Chinese Medicinal Materials

摘　　要：目的:观察三味檀香散对大鼠心肌缺血-再灌注损伤的病理组织学、脂质过氧化和抗氧化的影响。方法:采用结扎冠状动脉造成大鼠心肌缺血-再灌注的动物模型,实验大鼠随机分为伪手术组、缺血-再灌注模型组、阳性对照组、三味檀香散高、低剂量组,记录各组大鼠心电图ST段变化、测定血清乳酸脱氢酶(LDH)和磷酸肌酸激酶(CK)、心肌超氧化物歧化酶(SOD)、丙二醛(MDA)、谷胱甘肽过氧化物酶(GSH-Px)的变化,并留取心脏进行组织形态学观察。探讨三味檀香散对心肌缺血-再灌注损伤保护作用的机制。结果:高、低剂量的三味檀香散组大鼠心电图ST段移位明显低于模型组(P<0.05),亦能明显降低心肌LDH、CK的释放(P<0.05),可明显升高心肌SOD和GSH-Px活性,降低MDA含量(P<0.05),并且能明显减轻缺血-再灌注损伤区超微结构的病理改变。结论:藏药三味檀香散对缺血-再灌注损伤大鼠的心肌具有一定的保护作用,其机制可能与增强自由基清除剂的功能,维护心肌细胞氧化和抗氧化平衡,阻止脂质过氧化有关。Objective：To study the effect of Sanwei Tanxiang powder on myocardial pathologic change,myocardium lipid peroxidation and antioxidation on anesthetized rats′ hearts against myocardial ischemia and reperfusion injury.Methods：A model of regional myocardial ischemia-reperfusion injury（IR） was established by 30 min ligation of the left anterior descending coronary artery followed by 40 min reperfusion in hearts of anesthetized rats′.The experimental animals were randomly divided into the sham operation,IR model group,positive control group and two dose Sanwei Tanxiang powder groups.The changes of ECG,LDH,CK,SOD,GSH-Px and MDA in the heart were measured and ultrastructural pathological changes were observed.Results：In Sanwei Tanxiang groups the displacement of ST segment reduced（P〈0.05）,the activity of LDH,CK decreased（P〈0.05）,the activities of SOD,GSH-Px significantly increased and the contents of MDA reduced（P〈0.05）.Moreover,the development of ultrastructral pathological changes obviously improved compared with the IR model group.Conclusion：The protective effects of Sanwei Tanxiang powder on anesthetized rats′ hearts against myocardial ischemia and reperfusion injury may be related to protecting the antioxidants and inhibiting the lipid peroxidation in the myocardium.

关 键 词：三味檀香散 心肌缺血-再灌注 超微结构病理改变 脂质过氧化 

分 类 号：R285.5[医药卫生—中药学]