低氧去血清培养条件下半乳糖凝集素-1对大鼠星形胶质细胞增殖和细胞周期的影响  

Effect of galectin-1 on proliferation and cell cycle of astrocyte cultured in serum-free and low-oxygen condition

作　　者：王建平[1] 渠文生[2] 董星河 唐颖馨[1] 李昕[2] 刘春岭[2] 王伟[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院神经内科,武汉430030 [2]郑州大学第二附属医院神经内科,郑州450014 [3]濮阳油田职工医院,濮阳457000

出　　处：《郑州大学学报（医学版）》2008年第4期640-642,共3页Journal of Zhengzhou University(Medical Sciences)

基　　金：河南省高校杰出科研人才创新工程基金资助项目2007KUCX2005

摘　　要：目的:研究低氧去血清培养条件下半乳糖凝集素-1(Gal-1)对星形胶质细胞增殖和细胞周期的影响。方法:低氧去血清条件下体外培养大鼠星形胶质细胞,分别用高(10mg/L)、中(1mg/L)、低(0.1mg/L)剂量Gal-1干预。采用BrdU掺入法检测星形胶质细胞增殖,流式细胞术检测星形胶质细胞的细胞周期变化。结果:干预1d后,Gal-1高、中、低剂量组BrdU阳性细胞率均低于无干预对照组(P<0.05);干预3d后,Gal-1高剂量组BrdU阳性细胞率仍低于无干预对照组(P<0.05),中、低剂量组BrdU阳性细胞率与对照组比较,差异无统计学意义(P<0.05)。干预1d后,Gal-1高、中剂量组星形胶质细胞S期细胞比例降低,G0/G1细胞比例增高(P<0.05)。结论:Gal-1抑制星形胶质细胞的增殖和细胞周期进程,可能参与抑制脑缺血后的胶质疤痕形成。Aim ：To investigate the effects of galectin-1 （ Gal-1 ） on the proliferation and cell cycle of astrocyte in serum-free and low-oxygen culture. Methods：Rat astrocyte was incubated by serum-free culture media combined with 10,1 , 0. 1 mg/L Gal-1 ,respectively. Then,astrocytes were incubated with BrdU for 1 day to detect the proliferation, and the cell cycle was analyzed by flow cytometry. Results： Compared with the astrocytes cultured without Gal-1, the proporation of Br- dU（ ＋ ） astrocytes cultured with 10,1,0.1 mg/L Gal-1 for 1 day were lower（P 〈0.05）, that of astrocytes cultured with 10 mg/L Gal-1 for 3 days was still lower（P 〈0.05）. Cell cycle of astrocyte cultured with 10 and 1 mg/L Gal-I for 1 day was arrested with the decrease of S-phase cells. Conclusion：Galectin-1 can reduce the proliferation of astrocyte and arrest its cell cycle progress, which may be useful to reduce the formation of astroglia scar appearing after brain ischemia.

关 键 词：半乳糖凝集素-1 星形胶质细胞 增殖 细胞周期 大鼠 

分 类 号：R741.05[医药卫生—神经病学与精神病学]