人皮肤基底细胞癌和鳞状细胞癌组织中P27^(kip1)、Skp2蛋白的表达  

Expression of P27^(kip1) and Skp2 in human cutaneous basal cell carcinoma and squamous cell carcinoma tissue

作　　者：王璐[1] 郝媛媛[1] 刘林嶓[1] 马长路[2] 

机构地区：[1]郑州大学第一附属医院整形外科,郑州450052 [2]郑州大学第一附属医院病理科,郑州450052

出　　处：《郑州大学学报（医学版）》2008年第4期666-668,共3页Journal of Zhengzhou University(Medical Sciences)

基　　金：河南省科技攻关基金资助项目0124170636

摘　　要：目的:检测人皮肤基底细胞癌(BCC)和鳞状细胞癌(SCC)组织中P27kip1、Skp2的表达情况,并探讨其临床意义。方法:应用免疫组织化学SP染色法检测27例BCC(男15例,女12例;均无淋巴结转移)、23例SCC(男15例,女8例;高分化18例,中、低分化5例;均无淋巴结转移)和20例正常皮肤组织中P27kip1和Skp2蛋白的表达情况。结果:BCC和SCC组织中P27kip1的阳性表达率分别为63.0%和26.1%,低于正常皮肤组织的阳性表达率95.0%(P均<0.05)。BCC和SCC组织中Skp2的阳性表达率分别为40.7%和69.6%,高于正常皮肤组织的阳性表达率10.0%(P均<0.05)。BCC与SCC组织比较,2种蛋白阳性表达率差异均有统计学意义(P均<0.05)。BCC和SCC组织中,P27kip1与Skp2蛋白阳性表达率成负相关(P均<0.05)。结论:P27kip1低表达和Skp2高表达可能与BCC和SCC的发生、发展有关。Aim： To detect the expression of P27kip1 and Skp2 protein in cutaneous basal cell carcinoma（BCC） and squamous cell carcinoma（SCC） tissue. Methods：P27kip1 and Skp2 protein expression were detected in skin lesions of 27 cases of BCC（ 15 cases of male, 12 cases of female; all without lymphatic metastasis） , 23 cases of SCC （ 15 cases of male,8 cases of female; 18 cases of well differentiated, 5 case of moderately differentiated and poorly differentiated ; all without lymphatic metastasis） and 20 normal skin using immunohistochemistry. Results： Expression of P27kip1 protein was detected in a significantly lower proportion in BCC （63.0%） and SCC（26.1% ） than that in normal skin （95.0%） （P 〈 0.05 ）. Expression of Skp2 protein was detected in a significantly higher proportion in BCC （40.7%） and SCC （69.65%） than that in normal skin（ 10.0% ） （ P 〈 0.05 ）. There were significant difference between BCC and SCC on P27~ipl and Skp2 expression（ P 〈 0.05 ）. In BCC and SCC tissue, P27k^pl expression was negatively correlated with Skp2 （P 〈 0.05 ）. Conclusion： The abnormal expression of P27kip1 and Skp2 may contribute to the pathogenesis and progression of BCC and SCC.

关 键 词：皮肤肿瘤 基底细胞癌 鳞状细胞癌 激酶抑制蛋白-1 S期激酶相关蛋白-2 

分 类 号：R739.5[医药卫生—肿瘤]