白血病细胞源树突状细胞功能测定  

Antigen presenting function of dendritic cells derived from leukemia cells

作　　者：余莉[1] 戴育成[1] 李洁[1] 邹湉[1] 

机构地区：[1]南昌大学第二附属医院血液病研究所,南昌330025

出　　处：《郑州大学学报（医学版）》2008年第4期668-671,共4页Journal of Zhengzhou University(Medical Sciences)

基　　金：江西省卫生厅科研基金资助项目20051104

摘　　要：目的:观察体外诱导的白血病细胞源树突状细胞(DC)的抗原递呈功能。方法:分离初诊8例急性髓细胞性白血病(AML)、9例慢性髓细胞性白血病(CML)患者和6例正常人的骨髓单个核细胞,用rhGM-CSF、rhIL-4和TNF-α诱导培养。分别于培养第1、6、14天用倒置显微镜进行形态学观察,流式细胞术检测免疫学表型,用混合淋巴细胞反应检测抗原递呈功能。结果:经诱导培养,AML,CML和正常组骨髓单个核细胞均出现DC典型形态的细胞,而且免疫标记差异无统计学意义(P>0.05)。混合淋巴细胞反应结果显示,AML-DC、CML-DC和正常DC诱导生成的细胞毒T淋巴细胞(CTL)能够杀灭白血病细胞,AML-DC、CML-DC之间刺激T细胞增殖的能力相似(P>0.05),但2者刺激T细胞增殖的能力均低于正常DC(P<0.05)。结论:急性和慢性白血病细胞均能诱导分化成DC,但其抗原递呈功能较弱。Aim ： To observe the function of antigen presenting of dendritic cells （DCs） from primary acute myeloid leukemia（AML） and chronic myiloid leukemia（CML） cells. Methods：Bone marrow mononuclear cells（MNCs） were isola- ted from 8 patients with AML,9 patients with CML and 6 healthy donors, and co-cuhured with rhGM-CSF, rhlL-4 and TNF-ct. The morphologic features were observed by inverted microscope at Day 1,6, 14; CD80,CD86, CD1 a, HLA-DR ex- pression were assayed by flowcytometry, the function of antigen presenting were tested by mixed lymphocyte reaction （MLR）. Results： After cultured with cytokines, cells appearing the typical morphologic features and immunophentypes of DCs were founded in AML,CML and normal groups. The result of MLR showed that DCs of the three groups could stimulate the T cells to be CTL, which could kill the leukemia cells. The morphologic features, immunophentype expression and anti- gen presenting function of AML-DCs and CML-DCs did not differ from each other（ P 〉 O. 05 ） , but the antigen presenting function of AML-DCs and CML-DCs were weaker than that of the normal DCs. Conclusion： Primary AML and CML cells could be induced into AML-DCs and CML-DCs, but their antigen presenting function are weaker.

关 键 词：白血病 髓系 树突状细胞 细胞表型 

分 类 号：R733.7[医药卫生—肿瘤]